AI Article Synopsis
- The study investigates the relationship between secretory leucocyte protease inhibitor (SLPI) and Annexin A2 (AnxA2) in tonsillar cancer and HPV status, analyzing samples from patients with both conditions.
- Results show that smoking significantly increases SLPI expression, while HPV-positive samples exhibit lower SLPI and higher AnxA2 levels, suggesting smoking may block HPV from binding to AnxA2.
- The expression of SLPI and AnxA2 can be predicted through analysis of swabs and sputum, indicating potential for these tests to aid in preventing HPV-related cancers with interventions like tonsillectomy or vaccination.
Article Abstract
Previous retrospective studies have elucidated a correlation between secretory leucocyte protease inhibitor (SLPI) and Annexin A2 (AnxA2), patient smoking status and tonsillar human papilloma virus (HPV) status. The current study assessed these parameters prospectively and to the best of our knowledge, analyzed SLPI-/AnxA2-expression for the first time in tonsillar swabs and sputum. Samples were obtained from 52 patients with tonsillar squamous cell carcinoma and 163 patients with tonsillar hyperplasia (H; n=56) and chronic or recurrent tonsillitis (CRT; n=107). HPV-DNA, SLPI and AnxA2 gene expression was analyzed in sputum, tonsillar swabs and tissue by performing reverse transcription-quantitative PCR. Results were compared with smoking status, revealing that smoking resulted in significantly increased SLPI gene expression in all biomaterials of all cases. SLPI-gene expression was significantly decreased in all HPV-DNA-positive samples (tissue/swab/sputum), while AnxA2 was significantly increased in all HPV-DNA-positive samples. Results from swabs and sputum were able to predict SLPI- and AnxA2 gene expression of the corresponding tonsil. The current prospective study confirmed previous retrospective results underlining this hypothesis: Smoking enhances SLPI-expression, preventing HPV-binding to AnxA2. HPV-binding to AnxA2 appears essential for successful cell-entry. SLPI/AnxA2-gene expression in swabs and sputum reflect their expression in tonsillar tissue. Accordingly, a positive AnxA2/SLPI-ratio in sputum/swabs could possibly be used to reduce HPV-associated carcinogenesis, by performing tonsillectomy or HPV-vaccination in patients with positive AnxA2/SLPI-ratios.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988262 | PMC |
| http://dx.doi.org/10.3892/ol.2022.13284 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetic Concordance of Staphylococcus aureus From Oropharyngeal and Sputum Cultures in People With Cystic Fibrosis.
Pediatr Pulmonol
January 2025
Stead Family Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA.
Background: People with cystic fibrosis (CF) may not expectorate sputum at young ages or after they receive CFTR modulators. While oropharyngeal swabs are commonly used to test for lower airway pathogens, it is unknown whether Staphylococcus aureus from the oropharynx matches the strain(s) infecting the lungs. Our goal was to determine whether oropharyngeal and sputum isolates of S.
View Article and Find Full Text PDFRapid quantitative PCR on tongue swabs for pulmonary tuberculosis in adults: a prospective multicentre study.
Eur Respir J
January 2025
Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China
Background: Tuberculosis (TB) remains a major cause of infectious disease mortality globally, with significant underdiagnosis perpetuating transmission. Tongue swab analysis has emerged as a promising non-invasive method for pulmonary TB diagnosis. This study evaluates the diagnostic accuracy of the TB-EASY quantitative PCR (qPCR) assay using tongue swab specimens.
View Article and Find Full Text PDFHigh-sensitivity detection of DNA in tongue swab samples.
J Clin Microbiol
December 2024
Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
Article Synopsis
- Tongue swab (TS) sampling combined with quantitative PCR (qPCR) shows promise as an alternative to sputum testing for tuberculosis (TB) diagnosis, although previous studies indicated lower sensitivity.
- Two strategies were evaluated to enhance sensitivity: one involved centrifugation to concentrate MTB DNA, achieving 83% sensitivity and 100% specificity, while the other utilized sequence-specific magnetic capture, resulting in 90% sensitivity and 97% specificity.
- These findings suggest that with further development, these methods could lead to more accessible and effective TB testing options.
Changing profile of bacterial infection and microbiome in cystic fibrosis: when to use antibiotics in the era of CFTR-modulator therapy.
Eur Respir Rev
October 2024
Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany
The advent of cystic fibrosis transmembrane conductance regulator (CFTR) modulator therapy, especially the triple therapy combining the drugs elexacaftor, tezacaftor, ivacaftor (ETI), has significantly changed the course of the disease in people with cystic fibrosis (pwCF). ETI, which is approved for the majority (80-90%) of pwCF, partially restores CFTR channel function, resulting in improved mucociliary clearance and, consequently, improved lung function, respiratory symptoms and pulmonary exacerbations. The bacterial burden of classical CF pathogens such as and is reduced without reaching eradication in the majority of infected patients.
View Article and Find Full Text PDFPrevalence of Enterococcus Species in Various Clinical Samples and Their Antimicrobial Susceptibility Pattern.
Cureus
November 2024
Department of Microbiology, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, IND.
Article Synopsis
- Gram-positive enterococci, normally harmless gut bacteria, have become significant health threats, particularly to immunocompromised individuals, due to their ability to resist multiple antibiotics.
- An observational study involving 114 clinical samples (like urine and blood) aimed to assess the prevalence and resistance patterns of enterococci, highlighting a critical need for better infection control and surveillance measures.
- Results indicated that most isolates originated from urine (69.16%), with the highest infection rates found in individuals aged 41 to 50, emphasizing the varied distribution of these pathogens in clinical settings.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!