AI Article Synopsis
- The study addresses the challenge of monitoring exposure to harmful anthropogenic compounds through conventional methods that are limited to known chemicals.
- A new extraction process for human biomarkers in urine was developed that is less specific to individual compounds, allowing for a broader analysis.
- This method, combined with advanced mass spectrometry and data processing techniques, successfully identified 80% of tested compounds, offering a more comprehensive view of human exposure.
Article Abstract
Every day we are exposed to a cocktail of anthropogenic compounds many of which are biologically active and capable of inducing negative effects. The simplest way to monitor contaminants in a population is via human biomonitoring (HBM), however conventional targeted approaches require foreknowledge of chemicals of concern, often have compound specific extractions and provide information only for those compounds. This study developed an extraction process for human biomarkers of interest (BoE) in urine that is less compound specific. Combining this with an ultra-high resolution mass spectrometer capable of operating in full scan, and a suspect and non-targeted analysis (SS/NTA) approach, this method provides a more holistic characterization of human exposure. Sample preparation development was based on enzymatically hydrolysed urine spiked with 34 native standards and extracted by solid-phase extraction (SPE). HRMS data was processed by MzMine2 and 80% of standards were identified in the final data matrix using typical NTA data processing procedures.
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| http://dx.doi.org/10.1016/j.chemosphere.2022.134550 | DOI Listing |
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