The Role of Chitooligosaccharidolytic --Acetylglucosamindase in the Molting and Wing Development of the Silkworm .

Int J Mol Sci

State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Southwest University, Chongqing 400715, China.

Published: March 2022

The insect glycoside hydrolase family 20 --acetylhexosaminidases (HEXs) are key enzymes involved in chitin degradation. In this study, nine genes in were identified by genome-wide analysis. Bioinformatic analysis based on the transcriptome database indicated that each gene had a distinct expression pattern. qRT-PCR was performed to detect the expression pattern of the -- (). was highly expressed in chitin-rich tissues, such as the epidermis. In the wing disc and epidermis, has the highest expression level during the wandering stage. CRISPR/Cas9-mediated deletion was used to study the function. In the -knockout line, 39.2% of female heterozygotes had small and curly wings. The ultrastructure of a cross-section showed that the lack of affected the stratification of the wing membrane and the formation of the correct wing vein structure. The molting process of the homozygotes was severely hindered during the larva to pupa transition. Epidermal sections showed that the endocuticle of the pupa was not degraded in the mutant. These results indicate that is involved in chitin catabolism and plays an important role in the molting and wing development of the silkworm, which highlights the potential of as a pest control target.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998872PMC
http://dx.doi.org/10.3390/ijms23073850DOI Listing

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