Biofilm control by essential oil (EO) application has recently increased to preclude biofilm production on foods and environmental surfaces. In this work, the anti-biofilm effects of garlic and thyme essential oils using the minimum inhibitory concentration (MIC) method against recovered from different abattoir samples were investigated along with the virulence genes ( and genes), and the antimicrobial susceptibility profile of . as well. The obtained results revealed that . contaminated abattoir samples to varying degrees. The gene was investigated in all isolates, whereas the and genes were observed in four and three isolates, respectively. Utilizing the disc diffusion method, . isolates demonstrated substantial resistance to most of the examined antibiotics with a high multiple antibiotic resistance index. . isolates demonstrated biofilm formation abilities to various degrees at varied temperatures levels (4 °C and 37 °C). In conclusion, the obtained samples from the research area are regarded as a potential . contamination source. Furthermore, garlic essential oil (GEO) has more potential to inhibit . biofilm at different sub-minimum inhibitory concentrations as compared to thyme essential oil (TEO). Therefore, these EOs are considered as potential natural antibacterial options that could be applied in food industry.
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http://dx.doi.org/10.3390/molecules27072182 | DOI Listing |
Foods
January 2025
Center of Excellence Polymer Processing, Faculty of Engineering, Dunarea de Jos University of Galați, Domnească Street, No. 111, 800201 Galați, Romania.
Electrospinning is a versatile technique for obtaining nano/micro fibers which are able to significantly change the active properties of composite materials and bring in new dimensions to agri-food applications. Composite bio-based packaging materials obtained from whey proteins, functionalized with thyme essential oil (TEO) and reinforced by electrospun polylactic acid (PLA) fibers, represent a promising solution for developing new active food packaging using environmentally friendly materials. The aim of this study is to obtain and characterize one-side-active composite films covered with a PLA fiber mat: (i) WF/G1, WF/G2, and WF/G3 resulting from electrospinning with one needle at different electrospinning times of 90, 150, and 210 min, respectively, and (ii) WF/G4 obtained with two face-to-face needles after 210 min of electrospinning.
View Article and Find Full Text PDFPlants (Basel)
January 2025
Laboratory of Nanotechnology, Materials and Environment, Department of Chemistry, Faculty of Science, University Mohammed V, Rabat 10106, Morocco.
This study aimed to investigate the chemical composition and bioactivities of essential oils (EOs) from five Moroccan thyme species: subsp. , , subsp. , and .
View Article and Find Full Text PDFMolecules
December 2024
Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal.
Plant-derived essential oils (EOs) possess significant antimicrobial potential against spoilage and pathogenic microorganisms. However, their efficacy can vary depending on the test method, making it difficult to standardise results. This study aimed to investigate the effect of polysorbate 80, a common surfactant used to emulsify EOs, on antimicrobial activity and minimum inhibitory concentration (MIC) determinations.
View Article and Find Full Text PDFFood Chem
January 2025
Department Food Science, Federal University of Lavras, Lavras 37200-000, MG, Brazil.
Emulsions were prepared from T. vulgaris essential oil using the surfactants Pluronic F127 and Tween 80 by mechanical agitation (Emulsion_Tw and Emulsion_Pl) and sonication using an ultrasonic tip (Sonicated_emulsion_Tw and Sonicated_emulsion_Pl). These emulsions were incorporated into pectin films.
View Article and Find Full Text PDFReprod Toxicol
January 2025
Natural Resources Laboratory, University Mouloud Mammeri, BP 15017. Tizi-Ouzou. Algeria.
Pesticides tend to cause serious reproductive defects, disturbing endocrine functions and reducing fertility, especially in females. The objective of this work was to identify the reprotoxic effects of Ampligo® 150 ZC (AP), a mixture formulation of lambda cyhalothrin and chlorantraniliprole, on the ovary of female rabbits (Oryctolagus cuniculus) and the possible protective effect of co-treatment with thyme essential oil (TEO), extracted from (Thymus vulgaris) species, and vitamin C (vit C). Twenty female rabbits were divided into four equal groups (n=5): Control (distilled water), AP (20mg/ kg bw of the insecticide mixture every other day, by gavage for 28 days), AP+TEO (20mg/ kg bw of AP + 0.
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