Pollen identification is an important task for the botanical certification of honey. It is performed via thorough microscopic examination of the pollen present in honey; a process called melissopalynology. However, manual examination of the images is hard, time-consuming and subject to inter- and intra-observer variability. In this study, we investigated the applicability of deep learning models for the classification of pollen-grain images into 20 pollen types, based on the Cretan Pollen Dataset. In particular, we applied transfer and ensemble learning methods to achieve an accuracy of 97.5%, a sensitivity of 96.9%, a precision of 97%, an F1 score of 96.89% and an AUC of 0.9995. However, in a preliminary case study, when we applied the best-performing model on honey-based pollen-grain images, we found that it performed poorly; only 0.02 better than random guessing (i.e., an AUC of 0.52). This indicates that the model should be further fine-tuned on honey-based pollen-grain images to increase its effectiveness on such data.
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http://dx.doi.org/10.3390/plants11070919 | DOI Listing |
Ann Bot
December 2024
Instituto de Biologia, Universidade Federal de Uberlândia. Uberlândia, Brazil.
Background: Floral adaptations supposedly favour pollen grains to cross the numerous barriers faced during their journey to stigmas. Stamen dimorphism and specialized petals, like the cucculus in the Cassieae tribe (Fabaceae), are commonly observed in flowers that offer only pollen as a resource for bee pollinators. Here, we experimentally investigated whether the stamen dimorphism and cucculus enhance pollen placement on the bee's body.
View Article and Find Full Text PDFHardwareX
December 2024
School of Electrical Engineering and Computer Science, The Pennsylvania State University, University Park, 16802, PA, USA.
Honey bee foraging is a complex behavior because it involves tens of thousands of organisms making decisions about where to collect pollen and nectar based on the quality of resources and the distance to flowers. Studying this aspect of their biology is possible through direct observations but the large number of individuals involved in this behavior makes the implementation of technologies ideal to scale up this type of study. Consequently, there is a need for instruments that can facilitate accurate assessments of honey bee foraging at the colony level.
View Article and Find Full Text PDFBio Protoc
November 2024
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon1, CNRS, INRAE, Lyon, France.
In plants, the first interaction between the pollen grain and the epidermal cells of the stigma is crucial for successful reproduction. When the pollen is accepted, it germinates, producing a tube that transports the two sperm cells to the ovules for fertilization. Confocal microscopy has been used to characterize the behavior of stigmatic cells post-pollination [1], but it is time-consuming since it requires the development of a range of fluorescent marker lines.
View Article and Find Full Text PDFPlants (Basel)
November 2024
Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380000, Chile.
Honey is a natural sweet element that bees make with flower nectar, revered for its distinct flavor, nutritional value, and potential health benefits. Chilean beekeeping has a diverse range of honey varieties, many of which are unique. The quillay ( Molina, soapbark tree) is a Chilean endemic tree whose honey has not been studied in depth.
View Article and Find Full Text PDFPlants (Basel)
November 2024
Country Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.
With the rapid development of artificial intelligence, deep learning has been widely applied to complex tasks such as computer vision and natural language processing, demonstrating its outstanding performance. This study aims to exploit the high precision and efficiency of deep learning to develop a system for the identification of pollen. To this end, we constructed a dataset across 36 distinct genera.
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