E2f4 is a multifunctional transcription factor that is essential for many cellular processes. Although the role of E2f4 during cell cycle progression has been investigated in great detail, less is known about E2f4 during embryonic development. Here, we investigated the role of E2f4 during zebrafish development. Zebrafish e2f4 mutants displayed ectopic otolith formation due to abnormal ciliary beating in the otic vesicle. The beating defects of motile cilia were caused by abnormal expression of ciliary motility genes. The expression of two genes, lrrc23 and ccdc151, were significantly decreased in the absence of E2f4. In addition to that, e2f4 mutants also displayed growth retardation both in the body length and body weight and mostly died at around 6 months old. Although food intake was normal in the mutants, we found that the microvilli of the intestinal epithelia were significantly affected in the mutants. Finally, the intestinal epithelia of e2f4 mutants also displayed reduced cell proliferation, together with an increased level of cell apoptosis. Our data suggested a tissue-specific role of E2f4 during zebrafish development, which is distinct from the traditional views of this protein as a transcription repressor.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jcp.30734 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Defining epitranscriptomic hallmarks at the host-parasite interface and their roles in virulence and disease progression in Theileria annulata-infected leukocytes.
Biomed J
January 2025
Pathogen Genomics Group, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia; International Institute for Zoonosis Control, GI-CoRE, Hokkaido University, Sapporo, Japan. Electronic address:
Article Synopsis
- Theileria parasites lead to the transformation of bovine leukocytes, causing them to proliferate rapidly and evade cell death.
- The study investigates mA RNA modifications in T. annulata-infected leukocytes, using sequencing and bioinformatics to analyze these methylation patterns.
- Findings suggest that mA modifications correlate with leukocyte proliferation rates and may influence cell cycle dynamics, with HIF-1α identified as a potential regulator of these modifications, highlighting the importance of mRNA methylation in the interaction between the parasite and host cells.
Transcriptomic Analysis of HPV-Positive Oesophageal Tissue Reveals Upregulation of Genes Linked to Cell Cycle and DNA Replication.
Int J Mol Sci
December 2024
School of Life Sciences, Pharmacy and Chemistry, Kingston University London, London KT1 2EE, UK.
Human papillomavirus (HPV) is a prevalent sexually transmitted infection, implicated in various cancers, yet its influence in non-cancerous oesophageal tissue remains unclear. This study aims to investigate the gene expression changes associated with high-risk HPV (HR-HPV) in non-cancerous oesophageal tissue to elucidate potential early oncogenic mechanisms. Using RNA sequencing, we compared transcriptomic profiles of HPV-positive and HPV-negative non-cancerous oesophageal tissues.
View Article and Find Full Text PDFActin-based deformations of the nucleus control mouse multiciliated ependymal cell differentiation.
Dev Cell
December 2024
Institut de biologie de l'Ecole normale supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France. Electronic address:
Ependymal cells (ECs) are multiciliated cells in the brain that contribute to cerebrospinal fluid flow. ECs are specified during embryonic stages but differentiate later in development. Their differentiation depends on genes such as GEMC1 and MCIDAS in conjunction with E2F4/5 as well as on cell-cycle-related factors.
View Article and Find Full Text PDFNovel Core Gene Signature Associated with Inflammation-to-Metaplasia Transition in Influenza A Virus-Infected Lungs.
Int J Mol Sci
November 2024
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrent'ev Avenue 8, 630090 Novosibirsk, Russia.
Respiratory infections caused by RNA viruses are a major contributor to respiratory disease due to their ability to cause annual epidemics with profound public health implications. Influenza A virus (IAV) infection can affect a variety of host signaling pathways that initiate tissue regeneration with hyperplastic and/or dysplastic changes in the lungs. Although these changes are involved in lung recovery after IAV infection, in some cases, they can lead to serious respiratory failure.
View Article and Find Full Text PDFStudy of the Role of E2F1 and TMEM132A in Prostate Cancer Development.
Front Biosci (Landmark Ed)
October 2024
Department of Ultrasound, the Second Affiliated Hospital of Qiqihar Medical College, 161006 Qiqihar, Heilongjiang, China.
Objective: Identify transcription factors and target genes associated with prostate cancer, offering new therapy approaches.
Methods: Gene Set Enrichment Analysis (GSEA) investigates early 2 factor (E2F) transcription factor family roles in prostate cancer using the TCGA database. Survival analysis examined E2F factors and patient survival connections.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!