AI Article Synopsis
- Multiple attempts have been made to create T cells from pluripotent stem cells (PSCs), but these T cells often show low activity and poor immune function.
- The researchers outline a two-step method using an inducible PSC line (iR9-PSCs) that includes creating induced hematopoietic progenitor cells (iHPCs) in the lab and then transplanting them to develop functional T cells in living organisms.
- This process takes 21 days for iHPCs to prepare and an additional 4 to 6 weeks for the T cells to mature after transplantation, offering a straightforward and repeatable technique for generating functional T cells for research purposes.
Article Abstract
Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells using an inducible -PSC (iR9-PSCs) line. The procedure mainly includes generation of induced hematopoietic progenitor cells (iHPCs) in vitro, transplantation, and development of functional induced T cells (iT) in vivo via transplantation. The entire induction process in vitro requires 21 days before iHPCs transplantation. The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation. We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974948 | PMC |
| http://dx.doi.org/10.1097/BS9.0000000000000049 | DOI Listing |
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