Wound healing activity of -based topical formulations in mice.

Background And Aim: Ruiz and Pavón, a tuber native to South America, is characterized by its antioxidant, antimicrobial, and anti-inflammatory properties that contribute to wound healing. This study aimed to evaluate the healing effect of the topical formulations (gel and cream) on induced wounds in mice.

Materials And Methods: Here, an acidic ethanolic extract (1.5 N hydrochloric acid and 96% ethanol at the ratio of 15:85, v/v) was prepared with the tubers of ecotype black and incorporated into topical cream and gel formulations at 1%. Thirty-twoBalb/c mice were divided into four experimental groups receiving daily topical treatments for 14 days: Group I (control; no treatment), Group II (a commercial ointment of neomycin, polymyxin B, and bacitracin), Group III (1% gel), and Group IV (1% cream). The wound closure in the mice was determined during the treatment; then, they were euthanized to obtain skin samples for histopathological analysis.

Results: Groups III and IV showed a higher percentage of wound closure from the 6 day of the treatment. From the 8 day, the effect was greater in Group III. The healing effect was confirmed by the histopathological changes.

Conclusion: This study concludes that the topical formulations of demonstrate wound-healing activity in mice, and the most effective treatment is the 1% gel formulation.