A novel phenolphthalein-based fluorescent chemosensor for pyrophosphate detection via an Al displacement approach in real samples and living cells.

Spectrochim Acta A Mol Biomol Spectrosc

School of Chemistry and Chemical Engineering, and Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address:

Published: August 2022

Herein, we report a new phenolphthalein appended Schiff base (PASB) as reversible fluorescent sensor for the detection of pyrophosphate (PPi) ions through the metal displacement mechanism. PASB showed sensing exclusively toward Al ions in DMF/HO (v/v = 1/4, pH 5.5) solution, which resulted in a significant fluorescence enhancement at 540 nm. The 1: 2 binding stoichiometry for the complex formation between PASB and Al was confirmed by Job's plot and mass spectroscopic studies. Moreover, a solution of the in situ formed PASB-Al complex displayed a high selectivity to PPi. The addition of PPi to PASB-Al ensemble significantly quenched its fluorescence. Thus, a dual response was established based on "Off-On-Off" strategy for detection of both Al and PPi. The detection limit is 5.86 nM and 26 nM for Al and PPi, respectively. On this basis, we use PASB to detect Al in food samples. Furthermore, PASB was successfully applicable to detect Al and PPi for intracellular imaging in Human liver cancer cells.

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http://dx.doi.org/10.1016/j.saa.2022.121174DOI Listing

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