AI Article Synopsis
- The study aimed to explore the role of different immune cell types, specifically circulating follicular helper T cells (cTfh), extrafollicular helper T cells, and B cells, in chronic graft-versus-host disease (cGVHD) following stem cell transplantation.
- It was found that patients with cGVHD had significantly decreased levels of cTfh cells and increased levels of extrafollicular helper T cells compared to healthy donors, indicating a shift in immune response.
- Additionally, there were notable changes in B cell subsets, with higher percentages of antigen-stimulated B cells and a reduction in naive B cells, suggesting an altered immune landscape in cGVHD patients.
Article Abstract
Unlabelled: AbstractObjective: To investigate the role and mechanism of circulating follicular helper T cells (cTfh), extrafollicular helper T cells, B cells and their subsets in chronic graft-versus-host disease (cGVHD) after transplantation.
Methods: Peripheral blood of cGVHD 64 patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) at the First Affiliated Hospital of Soochow University from 2016 to 2019 were collected. The percentage of cTfh cells, extrafollicular helper T cells, B cells and subsets were detected by flow cytometry. The healthy donors were detected as control. Percentage of each cell subpopulation between the two groups were compared by the two-tailed Students' T test.
Results: The percentage of circulating follicular helper T cells (cTfh, CD4CXCR5) was markedly decreased in patients with cGVHD as compared with that in the healthy donors (0.53%±0.10% vs 3.91%±0.60%, P<0.001). The percentage of extrafollicular helper T cells (CD44CD62LPSGL-1CD4T) of the patients in cGVHD and the healthy donors were 8.86%±0.45% and 5.38%±0.79% (P=0.003). A significant change in B cell subsets was found in the patients with cGVHD. The two types of antigen-stimulate CD27 B cell: the percentages of pre-GC B cells (CD19IgDCD38CD27) and plasmablast/plasma cells (PB/PC, CD19IgDCD38CD27) of patients with cGVHD were 20.91%±2.70% and 41.05%±5.00%, respectively, which were significantly higher than those in healthy donors (P=0.005, P=0.014). Meanwhile, the percentage of unstimulated CD27 B cells in patients with cGVHD was significantly reduced, especially the naive B cells (CD19IgDCD38CD27, 12.59%±2.63%, P=0.025). There was a positive correlation between the percentage of extrafollicular helper T cells and plasmablast/ plasma cells (PB/PC) in cGVHD patients (r=0.43).
Conclusion: Compared with healthy donor, cTfh cells, extrafollicular helper T cells and B cell subsets in the peripheral blood of patients with cGVHD after transplantation changed in varying degress.
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| http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2022.02.045 | DOI Listing |
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