Cytogenetic and Molecular Characterization of (Characidae): A New Record of Supernumerary Chromosome in this Species.

The genus currently comprises 24 valid species distributed in the major river basins of South America. In this group, nine species were cytogenetically investigated, and found to share a diploid number of 50 chromosomes. Despite the conservation of the diploid number, variations in the karyotypic formula, number and position of the nucleolar organizer regions, and longitudinal bands have been described between both species and populations. In this study, we present cytogenetic and molecular data from specimens from the Keller River, a tributary of the Ivaí River (Upper Paraná basin), using DNA barcoding and cytogenetic markers (C-band, silver-stained nucleolar organizer regions, and fluorescence hybridization of 18S and 5S rDNA). The genetic inferences reached after analyzing the cytochrome oxidade subunit 1 gene allowed us to confirm the identity of the individuals with 2 = 50 chromosomes. However, one specimen contained a medium subtelocentric supernumerary chromosome (2 = 51). This is the second record of additional chromosomes in thereby confirming the existence of a supernumerary chromosome in allopatric populations of this species, a fact that demonstrates an evolutionary path that is divergent from other populations and/or species of analyzed so far, contributing to the karyotypic diversification of the group.