The oviduct of Chinese brown frog (Rana dybowskii) displays seasonal morphological and functional changes, which expands specifically during pre-brumation. To uncover the molecular mechanism underlying this phenomenon, we firstly confirmed the increased weight and enlarged diameter of the oviduct in pre-brumation by morphological observation. Interestingly, the glycogen content in the oviduct increased significantly during pre-brumation, indicating Rana dybowskii stores energy in the oviduct before brumation. Transcriptome analysis further identified the differentially expressed genes in the synthesis and metabolism pathways of carbohydrates in the oviduct during pre-brumation. Based on that evidence, we focused on the mRNA and protein expression of glycogenic genes in the oviduct of Rana dybowskii. qPCR confirmed that the expression of glycolysis and glycogenesis-related genes were up-regulated while gluconeogenesis-related genes were down-regulated during pre-brumation. Western blot data showed that glucose transporter GLUT1 and glycogen synthesis-regulation proteins including GYS, and p-GSK-3β were highly expressed in the oviduct during pre-brumation. Moreover, immunohistochemical data showed that GLUT1, GYS, p-GYS, GSK-3β and p-GSK-3β were expressed regionally in the oviduct of Rana dybowskii. The data suggests that glycogen synthesis may be involved in the oviductal expansion of Rana dybowskii during the pre-brumation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.theriogenology.2022.03.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Differential impact of wildfire and rice straw ash on the skin microbiota of Rana dybowskii adults and tadpoles.
Environ Pollut
December 2024
School of Biology and Agriculture, Jiamusi University, Jiamusi, 154007, China. Electronic address:
Global warming has intensified severe weather conditions and increased the frequency of wildfires, posing significant threats to ecosystems. Moreover, rice straw ash, a byproduct of human agricultural activities, represents an environmental stressor that can further impact these vulnerable ecosystems. These changes particularly impact sensitive species and microorganisms, yet limited research has explored the effects of wildfire ash and agricultural byproducts, such as rice straw ash, on amphibians.
View Article and Find Full Text PDFSynergistic toxicity of cadmium and triadimefon on the microbiota and health of Rana dybowskii tadpoles.
Comp Biochem Physiol C Toxicol Pharmacol
November 2024
School of Biology and Agriculture, Jiamusi University, Jiamusi 154007, China; Jiamusi Branch of Heilongjiang Academy of Forestry Sciences, Jiamusi 154002, China. Electronic address:
The skin and gut microbiota are crucial to amphibians. Triadimefon (TF), a widely used triazole fungicide, controls crop diseases and regulates growth, with uncertain effects on amphibian microbiota. Contamination, typically involving mixed chemicals at low concentrations, including cadmium (Cd) and TF, may detrimentally affect amphibian growth, survival, and microbiota health in both the skin and gut, but few research has examined these consequences.
View Article and Find Full Text PDFInfluence of wildfire ash concentration on development, survival, and skin and gut microbiota of Rana dybowskii.
Sci Total Environ
January 2025
Jiamusi Branch of Heilongjiang Academy of Forestry Sciences, Jiamusi 154002, China. Electronic address:
Article Synopsis
- Climate change is increasing wildfires, which threaten amphibian habitats and survival, but research on this connection is limited.
- This study examined the effects of wildfire ash on the gut and skin microbiota of Rana dybowskii tadpoles over 28 days, revealing that ash concentration significantly influenced body mass, development, and survival rates.
- The results indicated distinct changes in microbiota composition based on ash concentrations, showing that amphibians' microbiota are sensitive to environmental changes, highlighting the ecological impacts of wildfires on aquatic ecosystems.
Genome Survey of Male to Further Understand the Sex Determination Mechanism.
Animals (Basel)
October 2024
College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, China.
Article Synopsis
- - The study focuses on the genome analysis of a significant aquaculture species in Northeast China, which highlights the economic value of female individuals due to their use in traditional medicine.
- - Using next-generation sequencing, researchers obtained extensive genomic data, characterizing the genome's size, heterozygosity, and gene content, while generating 270,785 contigs and 498 scaffolds.
- - The research identifies homologies with male-specific DNA markers and suggests that there may be additional genes influencing the sex determination mechanism in this species, indicating complexity beyond previously recognized factors.
From Skin to Gut: Understanding Microbial Diversity in Rana amurensis and R. dybowskii.
Curr Microbiol
September 2024
College of Biology and Agriculture, Jiamusi University, Jiamusi, 154007, China.
Amphibians face the threat of decline and extinction, and their health is crucially affected by the microbiota. Their health and ecological adaptability essentially depend on the diverse microbial communities that are shaped by unique host traits and environmental factors. However, there is still limited research on this topic.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!