RNA-Seq Analysis Reveals the Potential Molecular Mechanisms of Puerarin on Intramuscular Fat Deposition in Heat-Stressed Beef Cattle.

Front Nutr

Jiangxi Province Key Laboratory of Animal Nutrition, Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, China.

Published: March 2022

AI Article Synopsis

  • The study aimed to explore how a specific dietary supplement affects intramuscular fat deposition in heat-stressed beef cattle, using 32 Jinjiang bulls divided into four groups based on supplement dosage.
  • Results indicated that the treatment increased crude fat and certain fatty acids, while reducing blood leptin levels, suggesting improved fat deposition.
  • High-throughput RNA sequencing revealed significant changes in gene expression related to lipid metabolism, highlighting the supplement's role in enhancing fat deposition under heat stress conditions.

Article Abstract

To investigate the effect of on intramuscular fat deposition in heat-stressed beef cattle and its underlying mechanism. Thirty-two healthy Jinjiang bulls were randomly divided into four groups and dietary with 0 (Control), 200 (Pue200), 400 (Pue400), and 800 (Pue800) mg/kg in the feed concentrate. The results showed that treatment enhanced the concentration of crude fat, fatty acid (C14:1 and C17:1), and the activity of fatty acid synthase in (LT), but decreased the levels of blood leptin ( < 0.05). High-throughput sequencing of mRNA technology (RNA-Seq) was used and the analysis showed that 492 genes were down-regulated and 341 genes were up-regulated in LT, and these genes were significantly enriched to the pathways related to lipid metabolism. These results indicated that dietary supplemental with enhanced intramuscular fat deposition by regulating lipid metabolism of heat-stressed beef cattle.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978796PMC
http://dx.doi.org/10.3389/fnut.2022.817557DOI Listing

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