AI Article Synopsis
- - The study aimed to evaluate the feasibility of FGFR3 testing and the frequency of its mutations in high-grade muscle invasive bladder cancer within the Brazilian healthcare system.
- - Out of 31 patients, 6 were excluded; RNA was successfully extracted from 84% of the samples, revealing FGFR3 mutations in 9.5% of analyzed cases, including specific mutations in two patients.
- - Recognizing the prevalence of FGFR3 mutations is crucial as it could inform treatment options and impact public health policies in Brazil.
Article Abstract
Objective: To understand the feasibility of FGFR3 tests in the Brazilian public health context, and to sample the mutational burden of this receptor in high-grade muscle invasive bladder cancer.
Methods: A total of 31 patients with high-grade muscle-invasive bladder cancer were included in the present study. Either transurethral resection of bladder tumor or radical cystectomy specimens were analyzed. Formalin-fixed paraffin-embedded tissue blocks were sectioned, hematoxylin and eosin stained, and histologic sections were reviewed. Total RNA was extracted using the RNeasy DSP formalin-fixed paraffin-embedded kit. Qualitative results were displayed in Rotor-Gene AssayManager software.
Results: Six patients were excluded. From the samples analyzed, four (16.7%) were considered inadequate and could not have their RNA extracted. Two patients presented FGFR3 mutations, accounting for 9.5% of material available for adequate analysis. The two mutations detected included a Y373C mutation in a male patient and a S249C mutation in a female patient.
Conclusion: FGFR3 mutations could be analyzed in 84% of our cohort and occurred in 9.5% of patients with high-grade muscle invasive bladder cancer in this Brazilian population. FGFR3 gene mutations are targets for therapeutic drugs in muscle-invasive bladder cancer. For this reason, know the frequency of these mutations can have a significant impact on public health policies and costs provisioning.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967311 | PMC |
| http://dx.doi.org/10.31744/einstein_journal/2022AO6450 | DOI Listing |
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