AI Article Synopsis
- Glaucoma is a major cause of blindness, and current treatments face issues like low effectiveness and poor patient adherence due to side effects.
- Researchers created bovine serum albumin nanoparticles (BSA-NPs) coated with chitosan (CS) to enhance the delivery of tetrandrine (TET) for glaucoma treatment.
- The optimized CS-BSA-NPs showed excellent drug encapsulation, improved corneal penetration, and biocompatibility, leading to a significant increase in drug bioavailability and sustained reduction in eye pressure in a rabbit model.
Article Abstract
Glaucoma is one of the leading causes of blindness. Therapies available suffer from several drawbacks including low bioavailability, repeated administration and poor patient compliance with adverse effects thereafter. In this study, bovine serum albumin nanoparticles (BSA-NPs) coated with chitosan(CS) were developed for the topical delivery of tetrandrine (TET) for glaucoma management. Optimized nanoparticles were prepared by desolvation. pH, BSA, CS and cross-linking agent concentrations effects on BSA-NPs colloidal properties were investigated. CS-BSA-NPs with particle size 237.9 nm and zeta potential 24 mV was selected for further evaluation. EE% exceeded 95% with sustained release profile. In vitro mucoadhesion was evaluated based on changes in viscosity and zeta potential upon incubation with mucin. transcorneal permeation was significantly enhanced for CS coated formulation. cell culture studies on corneal stromal fibroblasts revealed NPs biocompatibility with enhanced cellular uptake and improved antioxidant and anti-proliferative properties for the CS-coated formulation. Moreover, BSA-NPs were nonirritant as shown by HET-CAM test. Also, bioavailability in rabbit aqueous humor showed 2-fold increase for CS-TET-BSA-NPs compared to TET with a sustained reduction in intraocular pressure in a rabbit glaucoma model. Overall, results suggest CS-BSA-NPs as a promising platform for topical ocular TET delivery in the management of glaucoma.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004496 | PMC |
| http://dx.doi.org/10.1080/10717544.2022.2058648 | DOI Listing |
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