Thymus capitatus flavonoids inhibit infection of Kaposi's sarcoma-associated herpesvirus.

FEBS Open Bio

Laboratory of Epidemiology and Veterinary Microbiology, Institute Pasteur of Tunis, University of Tunis El Manar, Tunis-Belvedere, Tunisia.

Published: June 2022

Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpes virus 8 (HHV-8), causes primary effusion lymphoma, multicentric Castleman's disease, and Kaposi's sarcoma. Few antiviral drugs are available to efficiently control KSHV infection, and therefore, the development of novel, effective anti-KSHV treatments is needed. The aim of this study was to determine the antiviral activity of ethanolic and aqueous extracts, essential oils, and certain flavonoids (hesperidin, eupafolin, and vicenin) derived from Thymus capitatus (commonly known as thyme). We assessed the toxicity of these different extracts and components in RPE-1 cell cultures using the MTS test and evaluated their antiviral effect using the TCID method. The mechanism of action was determined through time-of-addition tests as well as viral entry, attachment, and virucidal assays. Additionally, western blot analysis was also used to assess their modes of action. Total treatment assay showed that the aqueous extract of T. capitatus has the highest inhibitory effect against KSHV with an EC value of 0.2388 µg·mL . Both hesperidin and eupafolin showed the ability to inactivate viral infection in a dose-response manner (EC values of 0.2399 and 1.396 µm, respectively). Moreover, they were able to inactivate KSHV postinfection by reducing viral protein expression. In summary, the effective antiviral property of the aqueous extract is likely a result of the inhibition of viral growth within the host cells by both hesperidin and eupafolin.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157407PMC
http://dx.doi.org/10.1002/2211-5463.13407DOI Listing

Publication Analysis

Top Keywords

hesperidin eupafolin
12
thymus capitatus
8
kaposi's sarcoma-associated
8
sarcoma-associated herpesvirus
8
aqueous extract
8
capitatus flavonoids
4
flavonoids inhibit
4
inhibit infection
4
infection kaposi's
4
herpesvirus kaposi's
4

Similar Publications

Thymus capitatus flavonoids inhibit infection of Kaposi's sarcoma-associated herpesvirus.

FEBS Open Bio

June 2022

Laboratory of Epidemiology and Veterinary Microbiology, Institute Pasteur of Tunis, University of Tunis El Manar, Tunis-Belvedere, Tunisia.

Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpes virus 8 (HHV-8), causes primary effusion lymphoma, multicentric Castleman's disease, and Kaposi's sarcoma. Few antiviral drugs are available to efficiently control KSHV infection, and therefore, the development of novel, effective anti-KSHV treatments is needed. The aim of this study was to determine the antiviral activity of ethanolic and aqueous extracts, essential oils, and certain flavonoids (hesperidin, eupafolin, and vicenin) derived from Thymus capitatus (commonly known as thyme).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!