Golf-shaped BiSe microparticles based-immunochromatographic strip for ultrasensitive detection of Acetamiprid.

J Hazard Mater

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China; Shenzhen Research Institute, Northwest A&F University, Shenzhen 518000, China. Electronic address:

Published: July 2022

Rapid and sensitive detection of pesticide is of significance to the field of food safety and human health, but it is still challenging due to interferents from complex food matrices. Herein, a superb golf-shaped BiSe microparticles-based immunochromatographic strip (BS MPs-ICS) was constructed for ultrasensitive detection of acetamiprid (ATM). The novel immune signal tag demonstrated outstanding luminance, excellent biocompatibility, and high affinity with ATM (affinity constant was 3.874 ×10 M), which not only possessed a preeminent labeling efficiency but also significantly improved detection sensitivity. After optimization, the limit of detection (LOD) of the BS MPs-ICS was 8.780 pg/mL with an excellent linear relationship at the range of 0.010-6.000 ng/mL, which was approximately 62-fold lower than that of conventional gold nanoparticles-ICS (0.545 ng/mL), The BS MPs-ICS biosensor was well applied in apple and tomato samples with satisfactory recoveries of 83.823-99.223% (relative standard deviation < 1.739%). Therefore, the BS MPs-ICS could serve as a promising candidate for ATM detection in complicated samples and develop a new method in real-time monitoring.

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Source
http://dx.doi.org/10.1016/j.jhazmat.2022.128810DOI Listing

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