Understanding physiologic and pathologic central nervous system function depends on our ability to map the entire cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels, iterative sample processing and micro-computed tomography, and automatic deformable image registration, feature extraction, and visualization. This methodology is applicable to any tissue and allows rapid exploration of normal and altered pathologic states.
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| http://dx.doi.org/10.1016/j.crmeth.2021.100151 | DOI Listing |
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