is the causative agent of listeriosis, a highly lethal disease initiated after the ingestion of -contaminated food. This species comprises different serovars, from which 4b, 1/2a, and 1/2b cause most of the infections. Among the different proteins involved in pathogenesis, the internalins A (InlA) and B (InlB) are the best characterized, since they play a major role in the enterocyte entry of cells during early infection. Due to their covalent attachment to the cell wall and location on the bacterial surface, along with their exclusive presence in the pathogenic , these proteins are also used as detection targets for this species. Even though huge advancements were achieved in the enrichment steps for subsequent detection, few studies have focused on the improvement of the antibodies for immunodetection. In the present study, recombinant InlA and InlB produced in were used as targets to generate antibodies via phage display using the human naïve antibody libraries HAL9 and HAL10. A set of five recombinant antibodies (four against InlA, and one against InlB) were produced in scFv-Fc format and tested in indirect ELISA against a panel of 19 strains (17 species; including the three main serovars of ) and 16 non- species. All five antibodies were able to recognize with 100% sensitivity (CI 29.24-100.0) and specificity (CI 88.78-100.0) in all three analyzed antibody concentrations. These findings show that phage display-derived antibodies can improve the biological tools to develop better immunodiagnostics for .

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964528PMC
http://dx.doi.org/10.3389/fpubh.2022.712657DOI Listing

Publication Analysis

Top Keywords

inla inlb
12
phage display-derived
8
inlb produced
8
antibodies
6
display-derived monoclonal
4
monoclonal antibodies
4
antibodies internalins
4
internalins allow
4
allow specific
4
specific detection
4

Similar Publications

L. monocytogenes is a Gram-positive bacterial pathogen, known to cause food poisoning and systemic disease, specifically listeriosis. This species has shown resistance to many commonly used antibiotics, making the search for new alternative therapies is a pressing matter.

View Article and Find Full Text PDF

The Impact of Subinhibitory Concentrations of Ɛ-polylysine, Hydrogen Peroxide, and Lauric Arginate on Listeria monocytogenes Virulence.

J Food Prot

December 2024

Dept. of Animal Science, University of Connecticut, Ag. Biotechnology Laboratory, 1390 Storrs Road, U-4163, Storrs CT, 06269-4163, USA. Electronic address:

Recent studies on the use of plant-derived and other bioactive compounds and antimicrobials in food have challenged the idea that exposure to antimicrobials at sublethal or subinhibitory concentrations (SICs) increases the virulence potential of bacterial pathogens including Listeria monocytogenes. The objective of this study was to determine the effect of exposure to SICs of Ɛ-polylysine (EPL), hydrogen peroxide (HP), and lauric arginate (LAE) on L. monocytogenes virulence.

View Article and Find Full Text PDF

Listeriosis is highly prevalent in the animal farming industry, with as the causative pathogen. To identify potential therapeutic targets for LM infection, we investigated the mechanisms of LM infection in goat uteri. We inoculated a group of goats with LM via jugular vein injection, isolated and raised them, and subsequently collected sterile samples of their uterine tissue after they exhibited clinical symptoms of LM infection.

View Article and Find Full Text PDF

Sanitizer Resistance and Persistence of Listeria monocytogenes Isolates in Tree Fruit Packing Facilities.

J Food Prot

October 2024

Department of Food Science, The Pennsylvania State University, University Park, PA 16802, United States. Electronic address:

Article Synopsis
  • The study focused on the persistence and antimicrobial resistance of the foodborne pathogen Listeria monocytogenes in three tree fruit packing facilities over two packing seasons (2020-2022).
  • Results showed that over half of the isolates had resistance to peroxyacetic acid (PAA) at concentrations similar to those used for sanitizing, while the majority were sensitive to a biofilm-removing agent at much lower concentrations.
  • Genomic analysis revealed that all isolates carried multiple antimicrobial resistance and virulence genes and demonstrated long-term persistence of certain genotypes at a specific facility over five years.
View Article and Find Full Text PDF

The mechanism of Enterococcus faecium on the virulence of Listeria monocytogenes during the storage of fermented sausages by whole genome analysis.

Int J Food Microbiol

September 2024

State Key Laboratory of Meat Quality Control and Cultured Meat Development, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, 210095 Nanjing, Jiangsu, PR China. Electronic address:

This study investigated the safety characteristics and potential probiotic properties of Enterococcus faecium by using whole genome analysis, and then explored the effect of this strain on the virulence of Listeria monocytogenes in vitro and during the storage of fermented sausages. Results showed that E. faecium B1 presented enterocin A, B, and P, enterolysin A, and UviB, and the exotoxin related genes and exoenzyme related genes were not detected in the genome of E.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!