Antibacterial Effect of Buds' Crude Extract Against Typhimurium and Potential Application in Cooked Chicken.

The objective of this study was to clarify the antibacterial activity and mechanism of buds' crude extract (CBCE) against Typhimurium, and explore the potential application in cooked chicken. The zone of inhibition (ZI), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were used to assess the antibacterial activity of CBCE against Typhimurium. The antibacterial mechanism was elucidated by revealing the changes in intracellular adenosine 5'-triphosphate (ATP) concentration, membrane potential, content of biomacromolecule, and cell morphology. Furthermore, the effect of CBCE on the counts of Typhimurium and color of cooked chicken during storage was studied. The results showed that the ZI, MIC, and MBC of CBCE against Typhimurium were 12.9 ± 0.53-13.6 ± 0.14 mm, 40, and 80 mg/mL, respectively. In the process of inhibiting Typhimurium by CBCE, the reduction of intracellular ATP concentration, cell membrane depolarization, leakage of protein and nucleic acid, and destruction of cell morphology were observed. Moreover, after treatments with CBCE, the growth of Typhimurium in cooked chicken was significantly inhibited ( < 0.05) compared with the control group. No significant differences ( > 0.05) in lightness (L*), redness (a*), and yellowness (b*) values of cooked chicken were found between untreated and treated samples, as well as the color of cooked chicken treated with CBCE did not change significantly ( > 0.05) during the six days of storage. Overall, our findings suggested that CBCE exhibited the antibacterial effect against Typhimurium, and had the potential to be used as a natural food preservative for the control of Typhimurium in chicken products.