The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(i)-catalysed alkyne-azide 'click' reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases on click-SELEX performance. We demonstrate click-SELEX being strongly dependent on which and on how many modifications are used. However, when using C3-GFP the number of modifications did not impact the overall success of the selection procedure.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905535 | PMC |
| http://dx.doi.org/10.1039/d2cb00012a | DOI Listing |
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