Advances in the field of stem cell-based models have in recent years lead to the development of blastocyst-like structures termed blastoids. Blastoids can be used to study key events in mammalian pre-implantation development, as they mimic the blastocyst morphologically and transcriptionally, can progress to the post-implantation stage and can be generated in large numbers. Blastoids were originally developed using mouse pluripotent stem cells, and since several groups have successfully generated blastocyst models of the human system. Here we provide a comparison of the mouse and human protocols with the aim of deriving the core requirements for blastoid formation, discuss the models' current ability to mimic blastocysts and give an outlook on potential future applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963787 | PMC |
| http://dx.doi.org/10.3389/fcell.2022.838356 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mouse totipotent blastomere-like cells model embryogenesis from zygotic genome activation to post implantation.
Cell Stem Cell
January 2025
MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Beijing Advanced Center of RNA Biology, Peking University, Beijing 100871, China. Electronic address:
Embryo development begins with zygotic genome activation (ZGA), eventually generating blastocysts for implantation. However, in vitro systems modeling the pre-implantation development are still absent and challenging. Here, we used mouse totipotent blastomere-like cells (TBLCs) to develop spontaneous differentiation and blastoid formation systems, respectively.
View Article and Find Full Text PDFEstablishment of bovine extraembryonic endoderm cells.
bioRxiv
December 2024
Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL 32610, USA.
Understanding the mechanisms of hypoblast development and its role in the implantation is critical for improving farm animal reproduction, but it is hampered by the lack of research models. Here we report that a chemical cocktail (FGF4, BMP4, IL-6, XAV939, and A83-01) enables de novo derivation and long-term culture of bovine extraembryonic endoderm cells (bXENs). Transcriptomic and epigenomic analyses confirmed the identity of bXENs and revealed that they are resemble hypoblast lineages of early bovine peri-implantation embryos.
View Article and Find Full Text PDFWDR36 Regulates Trophectoderm Differentiation During Human Preimplantation Embryonic Development Through Glycolytic Metabolism.
Adv Sci (Weinh)
December 2024
State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, Jiangsu, 211166, China.
Mammalian pre-implantation development is a complex process involving sophisticated regulatory dynamics. WD repeat domain 36 (WDR36) is known to play a critical role in mouse early embryonic development, but its regulatory function in human embryogenesis is still elusive due to limited access to human embryos. The human pluripotent stem cell-derived blastocyst-like structure, termed a blastoid, offers an alternative means to study human development in a dish.
View Article and Find Full Text PDFCapture of Totipotency in Mouse Embryonic Stem Cells in the Absence of Pdzk1.
Adv Sci (Weinh)
December 2024
State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai Animal Resources Center and Reproductive Regulation, Institute of Transplantation Medicine, Nankai University, Tianjin, 300350, China.
Totipotent cells can differentiate into three lineages: the epiblast, primitive endoderm, and trophectoderm. Naturally, only early fertilized embryos possess totipotency, and they lose this ability as they develop. The expansion of stem cell differentiation potential has been a hot topic in developmental biology for years, particularly with respect to the generation totipotent-like stem cells.
View Article and Find Full Text PDFNuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts.
Nat Commun
November 2024
Cell Fate Engineering and Therapeutics Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore, 138673, Republic of Singapore.
Article Synopsis
- Embryonic stem cells can self-organize into structures similar to blastocysts, which are important for studying early development and potential therapies.
- Researchers identified key factors influencing blastoid formation, focusing on the gene Nr1h2, which is critical for its development and operation across multiple species.
- Activation of Nr1h2 not only promotes blastoid creation but also enhances their ability to implant in the uterus and contribute to both embryonic and extraembryonic tissues, highlighting its broad regulatory role in early embryonic development.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!