AI Article Synopsis
- Scleractinians, or stony corals, are colonial organisms with strong abilities to regenerate and a complex immune system, making them ideal for studying the relationship between immunity and regeneration.
- Understanding this relationship is crucial for maintaining the health of coral reefs, which are vital ecosystems.
- The text outlines a protocol for using in situ hybridization with RNA probes to explore how immune factors are expressed in stony corals during their regeneration process.
Article Abstract
Scleractinians, or stony corals, are colonial animals that possess a high regenerative capacity and a highly diverse innate immune system. As such they present the opportunity to investigate the interconnection between regeneration and immunity in a colonial animal. Understanding the relationship between regeneration and immunity in stony corals is of further interest as it has major implications for coral reef health. One method for understanding the role of innate immunity in scleractinian regeneration is in situ hybridization using RNA probes. Here we describe a protocol for in situ hybridization in adult stony corals using a digoxigenin (DIG)-labeled RNA antisense probe which can be utilized to investigate the spatial expression of immune factors during regeneration.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9761507 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-2172-1_19 | DOI Listing |
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