Sponges (Porifera), basal nonbilaterian metazoans, are well known for their high regenerative capacities ranging from reparation of a lost body wall to whole-body regeneration from a small piece of tissues or even from dissociated cells. Sponges from different clades utilize different cell sources and various morphological processes to complete the regeneration. This variety makes these animals promising models for studying the evolution of regeneration in Metazoa. However, there are few publications concerning the regenerative mechanisms in sponges. This could be partially explained by the delicacy of sponge tissues, which requires modifying and fine adjusting of common research protocols. The current chapter describes various methods for studying regeneration processes in the marine calcareous sponge, Leucosolenia. Provided protocols span all significant research steps: from sponge collection and surgical operations to various types of microscopy and immunohistochemical studies.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9761524 | PMC |
http://dx.doi.org/10.1007/978-1-0716-2172-1_4 | DOI Listing |
Proc Natl Acad Sci U S A
December 2024
Department of Biological Sciences, University of Denver, Denver, CO 80208.
The discovery that sponges (Porifera) can fully regenerate from aggregates of dissociated cells launched them as one of the earliest experimental models to study the evolution of cell adhesion and allorecognition in animals. This process depends on an extracellular glycoprotein complex called the Aggregation Factor (AF), which is composed of proteins thought to be unique to sponges. We used quantitative proteomics to identify additional AF components and interacting proteins in the classical model, , and compared them to proteins involved in cell interactions in Bilateria.
View Article and Find Full Text PDFPeerJ
December 2024
Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
The sponge fauna of the Western Mediterranean stands as one of the most studied in the world. Yet sampling new habitats and a poorly studied region like the Balearic Islands highlights once again our limited knowledge of this group of animals. This work focused on demosponges of the order Tetractinellida collected in several research surveys (2016-2021) on a variety of ecosystems of the Balearic Islands, including shallow caves, seamounts and trawl fishing grounds, in a broad depth range (0-725 m).
View Article and Find Full Text PDFZootaxa
September 2024
The phylum Porifera has received limited attention in research studies along the Algerian coast. This study aims to compile a comprehensive inventory of Porifera species along the Algerian coast, spanning from the earliest documented work by Schmidt (1868) to the present day. The investigation meticulously catalogues a total of 136 species along the Algerian coast, distributed across 47 families, 18 orders, and 4 classes.
View Article and Find Full Text PDFZootaxa
October 2024
Dipartimento di Medicina Veterinaria; Università di Sassari; Via Vienna 2; 07100 Sassari; Italy.
A new occurrence of the genus Corvospongilla (Porifera: Demospongiae) is recorded from the Southeast Asia freshwater of Khorat Plateau, northeast Thailand. This is the second record of the genus from the Lower Mekong Basin with the discovery the new species Corvospongilla lampaoensis sp. nov.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!