[Analysis of CD11c B220NK cells in peripheral lymphoid tissues and the liver of C57BL/6 mice].

Objective To investigate the distribution of CD11cB220NK cells in peripheral lymphoid tissues and liver and the surface expression of plasmacytoid dendritic cell antigen-1 (PDCA-1) on CD11c B220 NK cells. Methods The spleen, lymph nodes and liver tissues of C57BL/6 mice were collected to prepare single-cell suspensions, and the proportion of CD11cB220NK cells in the tissues and their surface expression of PDCA-1 were detected by multi-color flow cytometry. Results CD11cB220NK cells were distributed widely in the spleen, lymph nodes and liver, with the highest proportion in the spleen (2.82±0.45)%. PDCA-1s were expressed in some of CD11cB220NK cells in the tissues, particularly in the spleen tissues. Conclusion CD11cB220NK cells are important subpopulation of NK cells in murine peripheral lymphoid tissues and liver. The expression of PDCA-1 on CD11cB220NK cells is different in different tissues.