AI Article Synopsis
- DNA in organisms, including humans, is structured into regions called topologically associating domains (TADs), which are important for gene regulation.
- Certain architectural proteins like BEAF-32, Cp190, Chro, and Dref are found at TAD borders, and their depletion alters TAD organization and chromatin looping, especially in areas with specific repressive modifications.
- The study shows that changes in TAD structures lead to differential expression of numerous genes, indicating that the architectural proteins play a crucial role in maintaining TAD organization and influencing gene activity.
Article Abstract
The DNA in many organisms, including humans, is shown to be organized in topologically associating domains (TADs). In , several architectural proteins are enriched at TAD borders, but it is still unclear whether these proteins play a functional role in the formation and maintenance of TADs. Here, we show that depletion of BEAF-32, Cp190, Chro, and Dref leads to changes in TAD organization and chromatin loops. Their depletion predominantly affects TAD borders located in regions moderately enriched in repressive modifications and depleted in active ones, whereas TAD borders located in euchromatin are resilient to these knockdowns. Furthermore, transcriptomic data has revealed hundreds of genes displaying differential expression in these knockdowns and showed that the majority of differentially expressed genes are located within reorganized TADs. Our work identifies a novel and functional role for architectural proteins at TAD borders in and a link between TAD reorganization and subsequent changes in gene expression.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997359 | PMC |
| http://dx.doi.org/10.1101/gr.275809.121 | DOI Listing |
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