AI Article Synopsis
- The study aimed to create a new method for assessing sperm quality by measuring mitochondrial oxygen metabolism in human sperm.
- Using normozoospermic samples, researchers optimized parameters for an extracellular flux analyzer to track oxygen consumption rates at different storage temperatures.
- Findings showed that while sperm motility decreased at lower temperatures (4°C) compared to 37°C, overall sperm vitality and oxygen consumption remained similar, indicating that OCR could be a reliable metric for sperm quality evaluation.
Article Abstract
Objective: We aimed to establish a novel sperm quality evaluation technology by measuring mitochondrial oxygen metabolism in human spermatozoa.
Results: Normozoospermic human sperm samples were used. After establishing the optimal parameters for measuring the oxygen metabolism of human sperm cells using the extracellular flux analyzer, we measured the oxygen consumption rate (OCR) of human spermatozoa exposed to different storage temperatures. Although sperm motility was significantly lower at 4 °C when compared with sperm motility at 37 °C, there were no significant differences in sperm vitality and the OCR under both conditions. The present study established a methodology for human sperm quality evaluation using extracellular flux analysis technology. The OCR evaluation could be a reliable measurement tool for assessing human sperm quality.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966288 | PMC |
| http://dx.doi.org/10.1186/s13104-022-06012-4 | DOI Listing |
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