This study aimed to evaluate the applicability of purified pea ingredients (starch and protein isolate) by assessing their potential to form volatile compounds during the different steps of sponge cake development compared to pea flour and wheat flour. While pea flour was highly susceptible to lipid oxidation during batter beating, the combination of purified pea starch and pea protein yielded significantly fewer oxidation markers with known green-beany off-odors. This was due more to the inactivation of lipoxygenase during flour fractionation than to differences in batter structure. However, fractionated ingredients were highly prone to participating in the Maillard reaction and caramelization during baking, leading to a more complex mixture of pyrazines, Strecker aldehydes and furanic compounds with potential malty and roasted notes compared to cakes based on pea flour or wheat flour. These findings confirm that using purified pea fractions can create high-quality products with an attractive composition.
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http://dx.doi.org/10.1016/j.foodchem.2022.132653 | DOI Listing |
Microb Cell Fact
December 2024
Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Cairo, Egypt.
Background: The main obstacle facing the utilization of microbial enzymes in industrial applications is the high cost of production substrates. As a result of the mentioned different wastes (coffee powder waste, dates nawah powder, molokhia stems, pea peels, lemon peels, and corn cobs) were investigated as low-cost nutritional substrates for the production of microbial β-galactosidase in this study. The purification of the enzyme and its kinetic and thermodynamics were investigated.
View Article and Find Full Text PDFPlant Dis
December 2024
INRAE Centre Bourgogne-Franche-Comté, 17 rue Sully, Dijon, France, 21000;
Root rot affects legumes such as lentil (Lens culinaris subsp. culinaris Medik.) and pea (Pisum sativum L.
View Article and Find Full Text PDFVirology
January 2025
Division of Biological and Biomedical Systems, School of Science and Engineering, University of Missouri-Kansas City, 5009 Rockhill Road, Kansas City, MO, 64110, USA. Electronic address:
Viral proteins with intrinsic disorder, such as the p26 movement protein from Pea enation mosaic virus 2 (PEMV2), can phase separate and form condensates that aid specific stages of virus replication. However, little is known about the impact of viral condensate formation on essential cellular processes, like translation. In this study, we performed mass spectrometry on affinity-purified p26 condensates and found an enrichment of RNA-binding proteins involved in translation and ribosome biogenesis.
View Article and Find Full Text PDFMolecules
October 2024
Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-779 00 Olomouc, Czech Republic.
Copper-containing diamine oxidases are ubiquitous enzymes that participate in many important biological processes. These processes include the regulation of cell growth and division, programmed cell death, and responses to environmental stressors. Natural substrates include, for example, putrescine, spermidine, and histamine.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address:
Bioactive urease from watermelon (Citrullus lanatus) seeds was purified using acetone fractionation, anion-exchange, and size-exclusion chromatography, achieving a 121-fold increase and specific activity of 3216 U/mg. The enzyme appeared as a single band on native and SDS-PAGE, with a molecular mass of 480 ± 10 kDa and subunit mass of 80 ± 10 kDa, indicating six identical subunits. Atomic absorption spectroscopy revealed 1.
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