Structure-Specific Glycoproteomics Characterization of NIST Monoclonal Antibody Reference Material 8671.

The characteristics of monoclonal antibodies (mAbs) cohering various function effectors show great expectation in therapy. Glycosylation, one of the common post-translational modifications, deeply influences cohesion. It is necessary to grasp monosaccharide composition/sequence and glycan structures in mAbs. There has been comprehensive mass spectrometry characterization of glycosylation of mAbs, and monosaccharide compositions are deduced according to known biosynthetic rules. Our recently developed intact glycopeptide search engine GPSeeker has made structure-specific characterization of glycosylation possible with structure-diagnostic fragment ions from selective fragmentation of glycan moieties. Here, we report our structure-specific glycoproteomics characterization of NIST monoclonal antibody reference material 8671 using GPSeeker, and 59 glycan structures (including 16 pairs of isomers) are characterized.