The steady-state kinetics of malate oxidation by malate dehydrogenase was being studied without coupling reagents under the conditions of chemical regeneration of the cofactor by the following pairs: phenazine methosulphate (PMS)--dichlorphenolindophenol (DCPIP) and PMS--tetranitrotetrazolium blue (TNTB). The comparative kinetic study was carried out of the steady-state oxidation of lactate and the reduction of pyruvate by lactate rehydrogenase, as well as of the dehydrogenation of lactate, coupled with the cofactor regeneration by the pair PMS-DCPIP. Optimum reagent concentrations, optimum pH and activation energies were determined for six systems. Malate dehydrogenation coupled with regeneration of the cofactor by the pair PMS-TNTB is the most promising reaction for enzyme immunoassay.
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