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Rapid quantitative PCR on tongue swabs for pulmonary tuberculosis in adults: a prospective multicentre study.
Eur Respir J
January 2025
Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China
Background: Tuberculosis (TB) remains a major cause of infectious disease mortality globally, with significant underdiagnosis perpetuating transmission. Tongue swab analysis has emerged as a promising non-invasive method for pulmonary TB diagnosis. This study evaluates the diagnostic accuracy of the TB-EASY quantitative PCR (qPCR) assay using tongue swab specimens.
View Article and Find Full Text PDFHigh-sensitivity detection of DNA in tongue swab samples.
J Clin Microbiol
December 2024
Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
Article Synopsis
- Tongue swab (TS) sampling combined with quantitative PCR (qPCR) shows promise as an alternative to sputum testing for tuberculosis (TB) diagnosis, although previous studies indicated lower sensitivity.
- Two strategies were evaluated to enhance sensitivity: one involved centrifugation to concentrate MTB DNA, achieving 83% sensitivity and 100% specificity, while the other utilized sequence-specific magnetic capture, resulting in 90% sensitivity and 97% specificity.
- These findings suggest that with further development, these methods could lead to more accessible and effective TB testing options.
USING SPUTUM AND TONGUE SWAB SPECIMENS FOR IN-HOME POINT-OF-CARE TARGETED UNIVERSAL TESTING FOR TB OF HOUSEHOLD CONTACTS: AN ACCEPTABILITY AND FEASIBILITY ANALYSIS.
medRxiv
November 2024
Desmond Tutu HIV Centre, University of Cape Town, Cape Town, South Africa.
Introduction: Effective strategies are needed to facilitate early detection and diagnosis of tuberculosis (TB). The overreliance on passive case detection, symptom screening, and collection of sputum, results in delayed or undiagnosed TB, which directly contributes to on-going TB transmission. We assessed the acceptability and feasibility of in-home, Targeted Universal TB Testing (TUTT) of household contacts using GeneXpert MTB/RIF Ultra at point-of-care (POC) during household contact investigations (HCIs) and compared the feasibility of using sputum vs.
View Article and Find Full Text PDFSputum and tongue swab molecular testing for the in-home diagnosis of tuberculosis in unselected household contacts: a cost and cost-effectiveness analysis.
medRxiv
October 2024
Desmond Tutu HIV Centre, University of Cape Town, Cape Town, South Africa.
Background: Delayed and missed diagnosis are a persistent barrier to tuberculosis control, partly driven by limitations associated with sputum collection and an unmet need for decentralized testing. Household contact investigation with point-of-care testing of non-invasive specimens like tongue swabs are hitherto undescribed and may be a cost-effective solution to enable community-based active case finding.
Methods: In-home, molecular point-of-care testing was conducted using sputum and tongue specimens collected from all household contacts of confirmed tuberculosis cases.
Non-sputum-based samples and biomarkers for detection of Mycobacterium tuberculosis: the hope to improve childhood and HIV-associated tuberculosis diagnosis.
Eur J Med Res
October 2024
Department of Medical Microbiology, College of Health Sciences Makerere University, Kampala, Uganda.
In 2014, the World Health Organisation (WHO) published target product profiles (TPP) for development of novel tuberculosis (TB) diagnostics. One of the key highlights is the need for point-of-care non-sputum-based tests capable of detecting all forms of TB through identification of characteristic biomarkers or biosignatures. Compared to the limitations associated with sputum-based TB tests, non-sputum samples are easy to collect, non-invasive, with potential to improve TB diagnosis among children and among people living with HIV/AIDS (PLHIV).
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