The biosynthetic gene cluster of atlantinone B () was discovered in MT-40. A multifunctional cytochrome P450 (AtlD) encoded by the cluster is responsible for the formation of the unique lactone-bridged ring and the 16β-hydroxyl of atlantinone B, and a new terpene cyclase (AtlC) can unprecedentedly accept the demethylated substrate epoxyfarnesyl-DMOA () to generate three bicyclic meroterpenoids (-). This study paves the way for combinatorial synthesis of structurally diverse meroterpenoids for drug discovery.
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