AI Article Synopsis
- The study explored the presence of nerve growth factor (NGF) in the kidneys of Swiss-Webster mice using immunocytochemical methods.
- NGF was found primarily in the apical and perinuclear cytoplasm of cells in the late distal nephron, specifically within the connecting tubule.
- Some intercalated cells in the connecting tubule showed no NGF presence, indicating variability in NGF distribution among these kidney cell types.
Article Abstract
The presence of NGF in mouse kidney was investigated using immunocytochemical methods. Female and male adult Swiss-Webster mouse kidneys were fixed by perfusion with 4% paraformaldehyde or Zamboni's fixative. The kidneys were frozen, and serial sections were prepared. Rabbit NGF antiserum was used for the primary incubation, and the avidin-biotin complex immunoperoxidase procedure was utilized for immunostaining. NGF immunoreactivity was localized in the apical and perinuclear cytoplasm of cells lining the late distal nephron, in a portion that corresponds to the connecting tubule. This portion of the nephron has been consistently observed in close anatomical relationship with arterioles, which are known to be richly innervated. Some cells of the connecting tubule, corresponding to intercalated cells, lacked NGF immunoreactivity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jnr.490160302 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography.
J Vis Exp
December 2024
Department of Ophthalmology and Visual Neurosciences, University of Minnesota;
G protein-coupled receptors (GPCRs) are a superfamily of transmembrane proteins that initiate signaling cascades through activation of its G protein upon association with its ligand. In all mammalian vision, rhodopsin is the GPCR responsible for the initiation of the phototransduction cascade. Within photoreceptors, rhodopsin is bound to its chromophore 11-cis-retinal and is activated through the light-sensitive isomerization of 11-cis-retinal to all-trans-retinal, which activates the transducin G protein, resulting in the phototransduction cascade.
View Article and Find Full Text PDFCalcineurin inhibitors (CNIs) are indispensable immunosuppressants for transplant recipients and patients with autoimmune diseases, but chronic use causes nephrotoxicity, including kidney fibrosis. Why inhibiting calcineurin, a serine/threonine phosphatase, causes kidney fibrosis remains unknown. We performed single-nucleus RNA sequencing of the kidney from a chronic CNI nephrotoxicity mouse model and found an increased proportion of injured proximal tubule cells, which exhibited altered expression of genes associated with oxidative phosphorylation, cellular senescence and fibrosis.
View Article and Find Full Text PDFDiabetic kidney disease (DKD) progression is often marked by early glomerular endothelial cell (GEC) dysfunction, including alterations in the fenestration size and number linked to impaired glomerular filtration. However, the cellular mechanisms regulating GEC fenestrations remain poorly understood due to limitations in existing models, challenges in imaging small fenestrations , and inconsistencies between and findings. This study used a logic-based protein-protein interaction network model with normalized Hill functions for dynamics to explore how glucose-mediated signaling dysregulation impacts fenestration dynamics in GECs.
View Article and Find Full Text PDFIntroduction: In USA, six million individuals with Sub-Saharan ancestry carry two high-risk variants, which increase the risk for kidney diseases. Whether APOL1 high-risk variants are independent risk factors for cardiovascular diseases is unclear and requires further investigation.
Methods: We characterized a mouse model to investigate the role of APOL1 in dyslipidemia and cardiovascular diseases.
Pharmacokinetics of Geraniol and Its Metabolites in Mice After Oral Administration.
Food Sci Nutr
January 2025
Aging Stress Response Research Project Team, National Center for Geriatrics and Gerontology Obu-city Aichi Japan.
Geraniol is an acyclic monoterpene alcohol that is extracted from the essential oils of aromatic plants. Geraniol has several biological activities such as anti-cancer, anti-inflammatory, antioxidant, and neuroprotective effects. However, the pharmacokinetics of geraniol and its metabolites after oral administration remain unknown in mice.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!