AI Article Synopsis
- The study focused on the genetic variability of HPV18 in Iran, identifying its prevalence and lineage distribution among a sample population from 2019 to 2021.
- Researchers analyzed 60 confirmed HPV18 cases, using molecular techniques to extract and amplify the E6 gene, successfully sequencing it for 43 samples.
- The results showed that sublineage A4 was the most common, comprising 69.7% of cases, followed by A1 and A3, while A2 and A5 were not detected, highlighting the need for further investigation into these sublineages and their impact on infection progression.
Article Abstract
The epidemiological studies in Iran on HPV18 nucleotide changes are rare. This type of virus is prevalent in the Iranian population. Therefore, in the present study, we aimed to identify the genetic variability in HPV18 in the E6 region to evaluate the prevalence of lineage distribution and sublineages in a sample population in Iran. Overall, 60 HPV18 confirmed cases were investigated between 2019 and 2021. The specimens were collected, and molecular genotyping was done using the Linear Array HPV Genotyping Test. DNA extraction was performed by a viral DNA/RNA kit. The HPV E6 gene was amplified by using type-specific primers designed according to the HPV18 genome prototype sequence. The sequencing of the E6 region was successfully done on 43 samples which were then compared to the reference sequence. The most frequent sublineage of HPV18 in this study was A4 (69.7%), followed by A1 (18.6%) and A3 (11.6%). Neither A2 nor A5 sublineage was not detected in this study. The related nucleotide acid changes according to the main references were as follows: A3: T104C/T232G/T485C/C549A, A4: T104C/T485C/C549A. The predominance of A lineage with the high frequency of A4 sublineage was found in the present research. The importance of sublineages in susceptibility to a progressive form of infection requires to be more investigated among the different population.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956376 | PMC |
| http://dx.doi.org/10.1155/2022/2839708 | DOI Listing |
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