Two novel bioassays useful for the quick assessment of chemical effects on the behavior of mosquito larvae (Culicidae) and adult earthworms (Lumbricidae).

The behavior, i.e., observable response of an organism can serve as a useful indicator of environmental quality. In the absence of death and other sublethal effects, behavioral changes can represent early warning signs of toxic effects. In the present contribution, we present two novel bioassays useful for the quick assessment of chemical effects on the behavior of mosquito larvae (Culicidae) and adult earthworms (Lumbricidae). These bioassays could have applications in ecological risk assessment and laboratory based ecotoxicological testing. The novel assays are:•A bioassay for the assessment of chemical toxicity on the swimming, breathing and resting behavior of Culicidae larvae.•A bioassay for the assessment of chemical toxicity on the escape behavior of Lumbricidae in water.•These bioassays were carried out using the systemic insecticide imidacloprid and documented the relatively rapid onset of the neurotoxic effects of imidacloprid to experimental organisms.