The Single-Nucleotide Polymorphism of miR-27a and the Expression of miR-27a in -Related Diseases and the Correlation with the Traditional Chinese Medicine Syndrome.

Aims: The study aims to explore the effects of the single-nucleotide polymorphism of miR-27a and its expression in -related diseases and the relationship between gastric pathology and traditional Chinese medicine (TCM).

Methods: Subjects were classified into six histopathological groups and five TCM syndrome groups. All specimens underwent detection through rapid urease test and methylene blue staining. Histopathological characteristics were observed by hematoxylin-eosin. The expression of miR-27a and its genotype were, respectively, detected by Quantitative Real-Time PCR and direct sequencing.

Results: promoted the malignant evolution of gastric mucosa and were involved in the formation of TCM syndrome. In -positive patients, the frequency of miR-27a CT genotype at the locus and its expression in the gastric cancer group were higher than those in other pathological groups. TCM syndrome had a close relationship with histopathological changes, and patients with spleen-qi deficiency syndrome had a higher risk of gastric cancer than other syndromes, regardless of infection.

Conclusion: The C allele at miR-27a locus may be an oncogene in gastric cancer. High levels of miR-27a could play an important role in gastric malignant evolution, especially cancerization. There is a certain connection between TCM syndrome and pathological changes of the gastric mucosa to some extent, where patients with SQD syndrome had a higher risk of GC.