AI Article Synopsis
- Intelectins are immune proteins found in chordates, including fish, that are activated during infections; however, their specific functions and properties in fish are not fully understood.
- The study focuses on zebrafish intelectin-2 (DrIntL-2), which is mainly found in the gastrointestinal tract, revealing its structure and binding characteristics with bacteria.
- Findings suggest DrIntL-2 could play a vital role in fish immune response and has potential applications in detecting and identifying harmful bacteria, benefiting the aquaculture industry.
Article Abstract
Intelectins are immune lectins expressed in chordates, including several fish species, in which intelectins are known to be upregulated upon infection. However, the basic biochemical properties and bacteria binding specificities of several fish intelectins are not well studied. We focus our investigation on zebrafish intelectin-2 (DrIntL-2) that is predominantly expressed in the gastrointestinal tract. The disulfide-linked oligomeric state and the cysteine responsible for intermolecular disulfide bonds are identified. DrIntL-2 is a globular particle of around 30 nm. In addition to the typical exocyclic 1,2-diol ligands, DrIntL-2 binds β-1,3-glucan and recognizes Salmonella typhimurium and Pseudomonas aeruginosa. This investigation not only shed light on the fish innate immunity that will be essential for the aquaculture industry, but will also provide a foundation for further application of DrIntL-2 in bacteria detection and identification.
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| http://dx.doi.org/10.1016/j.fsi.2022.03.025 | DOI Listing |
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