'Lamon bean' is a protected geographical indication (PGI) for a product of four varieties of bean ( L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E. of Italy). In the last decade, the 'Lamon bean' has been threatened by severe virus epidemics that have compromised its profitability. In this work, the full virome of seven bean samples showing different foliar symptoms was obtained by MinION sequencing. Evidence that emerged from sequencing was validated through RT-PCR and ELISA in a large number of plants, including different ecotypes of Lamon bean and wild herbaceous hosts that may represent a virus reservoir in the field. Results revealed the presence of bean common mosaic virus (BCMV), cucumber mosaic virus (CMV), peanut stunt virus (PSV), and bean yellow mosaic virus (BYMV), which often occurred as mixed infections. Moreover, both CMV and PSV were reported in association with strain-specific satellite RNAs (satRNAs). In conclusion, this work sheds light on the cause of the severe diseases affecting the 'Lamon bean' by exploitation of MinION sequencing.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951528PMC
http://dx.doi.org/10.3390/plants11060779DOI Listing

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'Lamon bean' is a protected geographical indication (PGI) for a product of four varieties of bean ( L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E.

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