'Lamon bean' is a protected geographical indication (PGI) for a product of four varieties of bean ( L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E. of Italy). In the last decade, the 'Lamon bean' has been threatened by severe virus epidemics that have compromised its profitability. In this work, the full virome of seven bean samples showing different foliar symptoms was obtained by MinION sequencing. Evidence that emerged from sequencing was validated through RT-PCR and ELISA in a large number of plants, including different ecotypes of Lamon bean and wild herbaceous hosts that may represent a virus reservoir in the field. Results revealed the presence of bean common mosaic virus (BCMV), cucumber mosaic virus (CMV), peanut stunt virus (PSV), and bean yellow mosaic virus (BYMV), which often occurred as mixed infections. Moreover, both CMV and PSV were reported in association with strain-specific satellite RNAs (satRNAs). In conclusion, this work sheds light on the cause of the severe diseases affecting the 'Lamon bean' by exploitation of MinION sequencing.
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http://dx.doi.org/10.3390/plants11060779 | DOI Listing |
Methods Mol Biol
December 2023
Department of Agriculture, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy.
The emergence of novel viral epidemics that could affect major crops represents a serious threat to global food security. The early and accurate identification of the causative viral agent is the most important step for a rapid and effective response to disease outbreaks. Over the last years, the Oxford Nanopore Technologies (ONT) MinION sequencer has been proposed as an effective diagnostic tool for the early detection and identification of emerging viruses in plants, providing many advantages compared with different high-throughput sequencing (HTS) technologies.
View Article and Find Full Text PDFPlants (Basel)
March 2022
Department of Agriculture, Food, Environmental and Animal Sciences, University of Udine, I-33100 Udine, Italy.
'Lamon bean' is a protected geographical indication (PGI) for a product of four varieties of bean ( L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E.
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