Interference with quorum-sensing (QS) intercellular communication systems by the enzymatic disruption of -acylhomoserine lactones (AHLs) in Gram-negative bacteria has become a promising strategy to fight bacterial infections. In this study, seven strains previously isolated from marine invertebrates and selected for their ability to degrade C6 and C10-HSL, were identified as , , , , and . AHL-degrading activity against a wide range of synthetic AHLs were identified by using an agar well diffusion assay and NTL4 and CV026 and VIR07 as biosensors. High-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis indicated that this activity was not due to an AHL lactonase. All the strains degraded AHLs in coculture experiments, while some strains reduced or abolished the production of virulence factors. In vivo assays showed that strains M3-111 and M3-127 reduced this pathogen's virulence and increased the survival rate of up to 3-fold, indicating its potential use for biotechnological purposes. To our knowledge, this is the first study to describe AHL-degrading activities in some of these marine species. These findings highlight that the microbiota associated with marine invertebrates constitute an important underexplored source of biological valuable compounds.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950658 | PMC |
| http://dx.doi.org/10.3390/microorganisms10030631 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bacteriological load in Holstein Friesian cows with dystocia.
Front Vet Sci
December 2024
Department Clinics, Reproduction, Faculty of Veterinary Medicine, University of Life Sciences (IULS), Iași, Romania.
The research took place on a farm in North-Eastern Romania with Holstein Friesian cows aged between 3 and 9 years. Bacteriological investigations were carried out throughout the year 2023, on a total of 35 cows, including 25 multiparous cows and 10 primiparous cows, 23 cows had eutocic parturitions and 12 cows had dystocic parturitions, during the first 3 weeks postpartum. In the case of dystocic parturition, biological samples yielded isolates including 9.
View Article and Find Full Text PDFMolecular characterization and antimicrobial susceptibility for 62 isolates of from children.
Front Microbiol
December 2024
Hebei Key Laboratory of Pathogens and Epidemiology of Infectious Diseases, HeBei Provincial Center for Disease Control and Prevention, Shijiazhuang, China.
Background: Pertussis is a highly contagious respiratory disease caused by (BP). Despite global control of pertussis cases through the Expanded Programme on Immunization (EPI), there has been a significant increase in the incidence of pertussis in recent years, characterized by a "resurgence" in developed countries with high immunization rates as well as a comparable reemergence in certain areas of China. We aim to explore the genotypes and antimicrobial susceptibility of circulating BP from children in Hebei.
View Article and Find Full Text PDFIsolation and characterization of a from fish pond water.
Front Microbiol
December 2024
Institute of Modern Aquaculture Science and Engineering (IMASE), Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, China.
Introduction: The intricate habitats of aquatic organisms, coupled with the prevalence of pathogens, contribute to a high incidence of various diseases, particularly bacterial infections. Consequently, the formulation of sustainable and effective disease management strategies is crucial for the thriving aquaculture sector.
Methods And Results: In this investigation, a strain of , designated , was isolated from a freshwater fish pond.
Genetic diversity and distinction of and in traditional Montenegrin brine cheeses and salamis.
Front Microbiol
December 2024
Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Vienna, Austria.
Introduction: is a widespread acid-lactic bacterium found in the environment, humans, and animal microbiota, and it also plays a role in the production of traditional food. However, the worldwide emergence of multidrug-resistant strains represents a major public health threat and is the primary reason that the genus is not recommended for the Qualified Presumption of Safety (QPS) list of the European Food Safety Authority (EFSA), raising concerns about its presence in food products.
Methods: In this study, 39 and 5 isolates were obtained from artisanal brine cheeses and dry sausages, sourced from 21 different Montenegrin producers.
In Vitro Screening of Bacterial Isolates From Dairy Products for Probiotic Properties and Other Health-Promoting Attributes.
Food Sci Nutr
December 2024
Paradise Scientific Company Ltd Dhaka Bangladesh.
The present research was aimed to isolate potential probiotic organisms from dairy products locally made in and around the Saurashtra region of Gujarat. A total of 224 colonies were screened for primary attributes. Based on the results, 70 isolates were carried further for secondary screening.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!