Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions.

Microorganisms

Laboratory of Microbiology and Probiotics, Institute of Nutrition and Food Technology (INTA), University of Chile, El Líbano 5524, Santiago 7830490, Chile.

Published: March 2022

AI Article Synopsis

  • Bacterial motility helps cells navigate towards better conditions and supports processes like colonization in hosts.
  • Different types of motility exist and are influenced by environmental factors, making realistic study methods crucial.
  • This text reviews common techniques for studying motility, categorizing them into macroscopic and microscopic approaches, and emphasizes the benefits of 3D imaging, particularly in host interaction studies using models like zebrafish.

Article Abstract

Bacterial motility is a widespread characteristic that can provide several advantages for the cell, allowing it to move towards more favorable conditions and enabling host-associated processes such as colonization. There are different bacterial motility types, and their expression is highly regulated by the environmental conditions. Because of this, methods for studying motility under realistic experimental conditions are required. A wide variety of approaches have been developed to study bacterial motility. Here, we present the most common techniques and recent advances and discuss their strengths as well as their limitations. We classify them as macroscopic or microscopic and highlight the advantages of three-dimensional imaging in microscopic approaches. Lastly, we discuss methods suited for studying motility in bacterial-host interactions, including the use of the zebrafish model.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953368PMC
http://dx.doi.org/10.3390/microorganisms10030563DOI Listing

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