Preparation of Mesoporous Silica Nanosphere-Doped Color-Sensitive Materials and Application in Monitoring the TVB-N of Oysters.

In this work, a new colorimetric sensor based on mesoporous silica nanosphere-modified color-sensitive materials was established for application in monitoring the total volatile basic nitrogen (TVB-N) of oysters. Firstly, mesoporous silica nanospheres (MSNs) were synthesized based on the improved Stober method, then the color-sensitive materials were doped with MSNs. The "before image" and the "after image" of the colorimetric senor array, which was composed of nanocolorimetric-sensitive materials by a charge-coupled device (CCD) camera were then collected. The different values of the before and after image were analyzed by principal component analysis (PCA). Moreover, the error back-propagation artificial neural network (BP-ANN) was used to quantitatively predict the TVB-N values of the oysters. The correlation coefficient of the colorimetric sensor array after being doped with MSNs was greatly improved; the Rc and Rp of BP-ANN were 0.9971 and 0.9628, respectively when the principal components (PCs) were 10. Finally, a paired sample -test was used to verify the accuracy and applicability of the BP-ANN model. The result shows that the colorimetric-sensitive materials doped with MSNs could improve the sensitivity of the colorimetric sensor array, and this research provides a fast and accurate method to detect the TVB-N values in oysters.