Anticancer and Anti-Neuroinflammatory Constituents Isolated from the Roots of .

Wasabi ( (Miq.) Matsum.) is a pungent spice commonly consumed with sushi and sashimi. From the roots of this plant, a new 2-butenolide derivative () and 17 previously reported compounds (-) were isolated and structurally characterized. Their chemical structures were characterized based on the conventional NMR (H and C, COSY, HSQC, and HMBC) and HRESIMS data analysis. All of these phytochemicals (-) were evaluated for their antiproliferative effects on the four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and MKN-1), for their inhibitory activity on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 microglia cells, and for their nerve growth factor (NGF)-releasing effect from C6 glioma cells. Among the isolated compounds, compound showed powerful antiproliferative activities against A549 and SK-MEL-2 cell lines with IC values of 2.10 and 9.08 μM, respectively. Moreover, the new compound exhibited moderate NO inhibition activity with IC value of 45.3 μM.