AI Article Synopsis
- * Fabrea salina has an extremely small macronuclear genome, the smallest among free-living heterotrophic eukaryotes, which shows streamlined characteristics such as high gene density and fewer introns.
- * The study delves into how Fabrea salina adapts to extreme environments, particularly high salt conditions, and suggests that its minimized genome can enhance our understanding of eukaryotic evolution and genome adaptation.
Article Abstract
Ciliated protists are among the oldest unicellular organisms with a heterotrophic lifestyle and share a common ancestor with Plantae. Unlike any other eukaryotes, there are two distinct nuclei in ciliates with separate germline and somatic cell functions. Here, we assembled a near-complete macronuclear genome of Fabrea salina, which belongs to one of the oldest clades of ciliates. Its extremely minimized genome (18.35 Mb) is the smallest among all free-living heterotrophic eukaryotes and exhibits typical streamlined genomic features, including high gene density, tiny introns, and shrinkage of gene paralogs. Gene families involved in hypersaline stress resistance, DNA replication proteins, and mitochondrial biogenesis are expanded, and the accumulation of phosphatidic acid may play an important role in resistance to high osmotic pressure. We further investigated the morphological and transcriptomic changes in the macronucleus during sexual reproduction and highlighted the potential contribution of macronuclear residuals to this process. We believe that the minimized genome generated in this study provides novel insights into the genome streamlining theory and will be an ideal model to study the evolution of eukaryotic heterotrophs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004412 | PMC |
| http://dx.doi.org/10.1093/molbev/msac062 | DOI Listing |
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