is a saprophytic fungus that can be found across the entire world. It can produce aflatoxin B (AFB), which threatens human health. CreA, as the central factor in carbon catabolite repression (CCR), regulates carbon catabolism and AFB biosynthesis in . Additionally, SsnF-RcoA are recognized as the corepressors of CreA in CCR. In this study, and not only regulated the expressions of CCR factors and hydrolase genes, but also positively affected mycelia growth, conidia production, sclerotia formation, and osmotic stress response in . More importantly, SsnF and RcoA were identified as positive regulators for AFB biosynthesis, as they modulate the AF cluster genes and the relevant regulators at a transcriptional level. Additionally, the interactions of SsnF-CreA and RcoA-CreA were strong and moderate, respectively. However, the interaction of SsnF and RcoA was weak. The interaction models of CreA-SsnF, CreA-RcoA, and SsnF-RcoA were also simulated with a docking analysis. All things considered, SsnF and RcoA are not just the critical regulators of the CCR pathway, but the global regulators involving in morphological development and AFB biosynthesis in .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954095 | PMC |
| http://dx.doi.org/10.3390/toxins14030174 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Corepressors SsnF and RcoA Regulate Development and Aflatoxin B Biosynthesis in NRRL 3357.
Toxins (Basel)
February 2022
Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
is a saprophytic fungus that can be found across the entire world. It can produce aflatoxin B (AFB), which threatens human health. CreA, as the central factor in carbon catabolite repression (CCR), regulates carbon catabolism and AFB biosynthesis in .
View Article and Find Full Text PDFRegulation of CreA-Mediated Catabolite Repression by the F-Box Proteins Fbx23 and Fbx47.
mBio
June 2018
Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Prêto, Bloco Q, Universidade de São Paulo, São Paulo, Brazil
The attachment of one or more ubiquitin molecules by SCF (kp-ullin--box) complexes to protein substrates targets them for subsequent degradation by the 26S proteasome, allowing the control of numerous cellular processes. Glucose-mediated signaling and subsequent carbon catabolite repression (CCR) are processes relying on the functional regulation of target proteins, ultimately controlling the utilization of this carbon source. In the filamentous fungus , CCR is mediated by the transcription factor CreA, which modulates the expression of genes encoding biotechnologically relevant enzymes.
View Article and Find Full Text PDFSCF Ubiquitin Ligase F-box Protein Fbx15 Controls Nuclear Co-repressor Localization, Stress Response and Virulence of the Human Pathogen Aspergillus fumigatus.
PLoS Pathog
September 2016
Department of Molecular Microbiology and Genetics and Göttingen Center for Molecular Biosciences (GZMB), Georg-August-University, Göttingen, Germany.
F-box proteins share the F-box domain to connect substrates of E3 SCF ubiquitin RING ligases through the adaptor Skp1/A to Cul1/A scaffolds. F-box protein Fbx15 is part of the general stress response of the human pathogenic mold Aspergillus fumigatus. Oxidative stress induces a transient peak of fbx15 expression, resulting in 3x elevated Fbx15 protein levels.
View Article and Find Full Text PDFRoles of the Aspergillus nidulans homologues of Tup1 and Ssn6 in chromatin structure and cell viability.
FEMS Microbiol Lett
December 2008
Instituto de Bioquímica Vegetal y Fotosíntesis (CSIC-Universidad de Sevilla), Centro de Investigaciones Científicas Isla de la Cartuja, Seville, Spain.
For three different carbon catabolite repressible promoters, alcA, alcR and the bidirectional promoter prnD-prnB, a deletion of rcoA, the Aspergillus nidulans homologue of TUP1, does not result in carbon catabolite derepression. Surprisingly, it results in disruption of the chromatin default structure of alcR and prnD-prnB promoters. In these promoters, and at variance with the wild type, repression occurs in the absence of nucleosome positioning.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!