Nonideal behavior of void volume markers in polar-organic chiral HPLC on polysaccharide-based chiral stationary phases.

J Liq Chromatogr Relat Technol

Chemistry Program, Department of Physical and Applied Sciences, University of Houston - Clear Lake, Houston, Texas, USA.

Published: August 2021

Polysaccharide-based chiral stationary phases account for a majority of chiral HPLC separations. Their behavior in normal-phase and reversed-phase modes of separation has been studied extensively. However, much less is known about their behavior in polar-organic mode. In the course of our studies on preparative separation of various cyclic imides, lactams, and acetamides on Chiralcel OD, Chiralcel OJ, Chiralpak AD, and Chiralpak AS stationary phases we sought to determine the void volumes of these columns using commonly used markers, LiNO, thiourea, and acetone. We were surprised to notice extensive column- and solvent-dependent deviations from ideal (unretained and unexcluded) behavior. These deviations likely result from multiple types of noncovalent interactions possible for these chiral stationary phases. Therefore, great care should be taken when choosing void volume markers for such separations. Our results indicate that with acetonitrile as a mobile phase, acetone is a suitable void volume marker for all four of the above stationary phases.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8939000PMC
http://dx.doi.org/10.1080/10826076.2021.1960858DOI Listing

Publication Analysis

Top Keywords

stationary phases
20
void volume
12
chiral stationary
12
volume markers
8
chiral hplc
8
polysaccharide-based chiral
8
chiral
5
stationary
5
phases
5
nonideal behavior
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!