A novel lateral flow assay (LFA) was developed based on the aggregation induced emission (AIE) of gold nanoclusters (AuNCs) for the detection of melamine. We compared the interaction between melamine and AuNCs prepared with 6-Aza-2-thiothymine (ATT) and its four structural analogs as ligands and found that the ATT-AuNCs showed the best AIE induced by melamine. Then the ATT-AuNCs were directly immobilized on the test line of LFA strips as reporters. The sensing platform exhibited a linear response to melamine from 1 to 100 μM (R = 0.997) with a detection limit of 217 nM. Moreover, this LFA strip could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min. The recoveries of 91.6-101.5% were attained in milk and baby formula samples. This strip inherited the superior characteristics of low background interference of aggregation-induced fluorescence recognition and LFA technology, thus effectively avoiding the use of antibodies, aptamers, and other biological elements.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.foodchem.2022.132670 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dual-mode CRISPR/Cas12a-assisted fluorescent and lateral flow aptasensor based on a newly truncated aptamer for Fumonisin B1 detection.
Int J Biol Macromol
January 2025
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address:
As a Group 2B carcinogen, accurate and efficient detection for Fumonisin B1 (FB1) is essential. The emergence of aptamers presents a viable solution to meet this demand. In this study, a truncated aptamer named Apt40 was developed, showcasing remarkable binding affinity to FB1.
View Article and Find Full Text PDFDevelopment of a rapid antigen-based lateral flow assay for tick-borne spotted fever rickettsioses.
PLoS One
January 2025
Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America.
Tick-borne spotted fever rickettsioses (SFRs) continue to cause severe illness and death in otherwise-healthy individuals due to lack of a timely and reliable diagnostic laboratory test. We recently identified a diagnostic biomarker for SFRs, the putative N-acetylmuramoyl-l-alanine amidase RC0497. Here, we developed a prototype laboratory test that targets RC0497 for diagnosis of SFRs.
View Article and Find Full Text PDFHemodynamic Relevance Evaluation of Coronary Artery Anomaly During Stress Using FFR/IVUS in an Artificial Twin.
JACC Case Rep
January 2025
Department of Cardiology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Anomalous aortic origin of coronary artery can lead to ischemia. Due to the limitations of invasive catheterization dobutamine stress testing, an alternative noninvasive approach is desired. A 65-year-old woman with atypical chest pain was referred for coronary computed tomography angiography.
View Article and Find Full Text PDFUnderstanding glucose metabolism and insulin action at the blood-brain barrier: Implications for brain health and neurodegenerative diseases.
Acta Physiol (Oxf)
February 2025
Research Center, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, China.
The blood-brain barrier (BBB) is a highly selective, semipermeable barrier critical for maintaining brain homeostasis. The BBB regulates the transport of essential nutrients, hormones, and signaling molecules between the bloodstream and the central nervous system (CNS), while simultaneously protecting the brain from potentially harmful substances and pathogens. This selective permeability ensures that the brain is nourished and shielded from toxins.
View Article and Find Full Text PDFRecent advances in rapid detection of Helicobacter pylori by lateral flow assay.
Arch Microbiol
January 2025
Department of Laboratory Medicine, Jinan Second People's Hospital of Shandong Province (Jinan Eye Hospital), No. 148, Jingyi Road, Jinan, 250022, Shandong, China.
Infection with H. pylori (Helicobacter pylori) is the most prevalent human infection worldwide and is strongly associated with many gastrointestinal disorders, including gastric cancer. Endoscopy is mainly used to diagnose H.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!