microRNAs (miRNAs) regulate target gene expression by pairing to target mRNAs, leading to mRNA degradation or translation inhibition. Out of several miRNAs in Arabidopsis, miR397b and miR857 regulate secondary growth by modulating lignin polymerization and deposition in secondary xylem cells by targeting laccases. Interestingly, the phytohormone ethylene is also suggested to have a role in lignin biosynthesis in tension wood formation. Despite this information, it is not known whether ethylene has any role in controlling secondary growth via miRNAs-mediated pathways. In this study, we elucidate that ethylene acts upstream to the miR397b/miR857-laccases module and negatively regulates lignin biosynthesis by directly activating the expression of both the miRNAs. The binding of EIN3 to the promoter of miR397b is further validated by yeast one-hybrid assay. In addition to its role in lignification, ethylene also regulates leaf serration by directly regulating the expression of NAC transcription factors, like CUP-SHAPED COTYLEDON2 (CUC2) and CUC3. Together, our study suggests a novel mechanism involving ethylene and miRNAs in lignin biosynthesis and leaf serration in Arabidopsis thaliana.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2022.03.037 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The role of lignin in 17β-estradiol biodegradation: insights from cellular characteristics and lipidomics.
Microb Cell Fact
December 2024
College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
17β-estradiol (E2) is an endocrine disruptor, and even trace concentrations (ng/L) of environmental estrogen can interfere with the endocrine system of organisms. Lignin holds promise in enhancing the microbial degradation E2. However, the mechanisms by which lignin facilitates this process remain unclear, which is crucial for understanding complex environmental biodegradation in nature.
View Article and Find Full Text PDFForage sources in total mixed rations on rumen fermentation, gut fill, and development of the gastrointestinal tract of dairy calves.
Sci Rep
December 2024
Department of Animal Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Av. Pádua Dias, n 11, Piracicaba, SP, 1341-900, Brazil.
The inclusion of forage sources in calf diets is often discussed, and the main point debated is whether the inclusion level, particle size, source, and how forage is offered may impact gut fill and reduce body weight gain, as well as impact gastrointestinal tract development. This study aimed to determine the effects of feeding forage sources with different qualities on rumen fermentation, gut fill, and development of the gastrointestinal tract of dairy calves. Forty-eight Holstein dairy calves were blocked according to sex and body weight (BW) at 28 days of life and randomly assigned to 1 of 4 dietary treatments.
View Article and Find Full Text PDFWhole genome analysis of 26 bacterial strains reveals aromatic and hydrocarbon degrading enzymes from diverse environmental soil samples.
Sci Rep
December 2024
Division of Research, Innovation, and Economic Development (RIED), Tarleton State University, Stephenville, TX, 76402, USA.
Polycyclic aromatic compounds and petroleum hydrocarbons (PHs) are hazardous pollutants and seriously threaten the environment and human health. However, native microbial communities can adapt to these toxic pollutants, utilize these compounds as a carbon source, and eventually evolve to degrade these toxic contaminants. With this in mind, we isolated 26 bacterial strains from various environmental soil samples.
View Article and Find Full Text PDFMicrobiological Aspects and Enzymatic Characterization of L7: Ascomycete with Great Biomass Degradation Potentialities.
J Fungi (Basel)
November 2024
Dipartmento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, 90133 Palermo, Italy.
The complex structure of the plant cell wall makes it difficult to use the biomass produced by biosynthesis. For this reason, the search for new strains of microorganisms capable of efficiently degrading fiber is a topic of interest. For these reasons, the present study aimed to evaluate both the microbiological and enzymatic characteristics of the fungus L7strain.
View Article and Find Full Text PDFGenetic improvement of low-lignin poplars: a new strategy based on molecular recognition, chemical reactions and empirical breeding.
Physiol Plant
December 2024
Laboratory of Tumor Targeted and Immune Therapy, Clinical Research Center for Breast, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, China.
As an important source of pollution in the papermaking process, the presence of lignin in poplar can seriously affect the quality and process of pulping. During lignin synthesis, Caffeoyl-CoA-O methyltransferase (CCoAOMT), as a specialized catalytic transferase, can effectively regulate the methylation of caffeoyl-coenzyme A (CCoA) to feruloyl-coenzyme A. Targeting CCoAOMT, this study investigated the substrate recognition mechanism and the possible reaction mechanism, the key residues of lignin binding were mutated and the lignin content was validated by deep convolutional neural-network model based on genome-wide prediction (DCNGP).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!