Development of SSR and SNP markers for identifying opium poppy.

Int J Legal Med

Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, People's Republic of China.

Published: September 2022

AI Article Synopsis

  • Papaver somniferum L. (opium poppy) is the source of heroin and poses significant health risks, leading to challenges in enforcing bans on its cultivation due to high profitability.
  • The study developed a set of eleven SSR markers and two SNP markers to effectively differentiate opium poppy from six other related Papaver species.
  • A new multiplex PCR system was created to enhance the efficiency of this identification process, which can aid in tackling illegal opium poppy cultivation and trafficking.

Article Abstract

Papaver somniferum L. (opium poppy) is the original plant of heroin, which is a major narcotic drug, and this plant has brought great harm to human health. However, the ban on opium poppy cultivation and trafficking is facing great challenges because of abnormal profits. Therefore, rapid and accurate identification is important to address the abovementioned problems. In this study, eleven simple sequence repeats (SSR) markers and two single nucleotide polymorphism (SNP) markers were mined to distinguish opium poppy from other six Papaver species. These molecular markers were further verified through a large number of plant materials of these seven Papaver species. An excellent multiplex polymerase chain reaction (PCR) system that simultaneously amplifies the three of eleven SSR markers was developed, which effectively improves the efficiency and speed of identification. The present research is of great implication for identifying and investigating the illegal cultivation and trafficking of opium poppy.

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Source
http://dx.doi.org/10.1007/s00414-022-02810-4DOI Listing

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