sp. nov. and sp. nov., two bacteria from constructed wetland.

Two Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strains designated 3A5MI-3 and RSS-23 were isolated from the Dragon-shaped Wetland System in Beijing Olympic Park, PR China. Strain 3A5MI-3 grew at 15–45 °C, pH 5.0–9.0 and with 0–2 % NaCl (w/v), and strain RSS-23 grew at 15-40 C, pH 5.5–9.0 and with 0–1 % NaCl (w/v). Phylogenetic analyses of 16S rRNA gene sequences revealed that strains 3A5MI-3 and RSS-23 were members of and , respectively. Phylogenetically closest relatives of strains 3A5MI-3 and RSS-23 were R384 and SY21, respectively. The cells of strain 3A5MI-3 contained menaquinone MK-7 and phosphatidylethanolamine, and the major cellular fatty acids were composed of iso-C, iso-C ω6 and/or iso-C ω7, iso-C 3-OH, C and summed feature 3 (C ω7/C ω6). Strain RSS-23 contained ubiquinone Q-8 and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unknown phospholipids and an unknown lipid, and its major cellular fatty acids were iso-C, iso-C ω9, iso-C 3-OH and summed feature 3 (C ω7/C ω6). DNA sequencing resulted in 6.59 Mb for the strain 3A5MI-3 genome and 2.79 Mb for the strain RSS-23 genome. The calculated G+C molar contents for strains 3A5MI-3 and RSS-23 were 47.07 and 61.21 mol%, respectively. According to phenotypic and phylogenetic characteristics, strains 3A5MI-3 and RSS-23 represent novel species of the genera and for which the names sp. nov. and sp. nov. are proposed. The type strain for sp. nov. is 3A5MI-3 (=CGMCC 1.17737=KCTC 82817). The type strain for sp. nov. is RSS-23 (=CGMCC 1.17738=KCTC 82820).