Background: Indigo-containing plant tissues change blue after a freezing treatment, which is accompanied by changes in indigo and its related compounds. is one of the few monocot plants containing indigo. The change to blue after freezing was described to explore the biosynthesis of indigo in .
Methods: In this study, we surveyed the dynamic change of flower metabolomics and transcriptomics.
Results: The non-targeted metabolomics and targeted metabolomics results revealed a total of 98 different metabolites, the contents of indole, indican, indigo, and indirubin were significantly different after the change to blue from the freezing treatment. A transcriptome analysis screened ten different genes related to indigo upstream biosynthesis, including three anthranilate synthase genes, two phosphoribosyl-anthranilate isomerase genes, one indole-3-glycerolphosphate synthase gene, five tryptophan synthase genes. In addition, we further candidate 37 cytochrome P450 enzyme genes, one uridine diphosphate glucosyltransferase gene, and 24 -D-glucosidase genes were screened that may have participated in the downstream biosynthesis of indigo. This study explained the changes of indigo-related compounds at the metabolic level and gene expression level during the process of under freezing and provided new insights for increasing the production of indigo-related compounds in . In addition, transcriptome sequencing provides the basis for functional verification of the indigo biosynthesis key genes in .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929171 | PMC |
| http://dx.doi.org/10.7717/peerj.13106 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enzymatic Antioxidants and Their Differential Response to Cryopreservation in Japanese Quail Semen.
Reprod Domest Anim
January 2025
Department of Zoology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, Rawalpindi, Pakistan.
A triad of enzymatic antioxidants viz., catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) constitutes a first line of defence against any redox imbalances in the semen. Cryopreservation enabling long term storage of semen also prompts generation of surplus reactive oxygen species (ROS) in the cells with waned antioxidants, hampering the full exploitation of this process.
View Article and Find Full Text PDFStrategy for enhancing Cistanche deserticola drying efficiency and quality based on novel freeze-infrared hybrid drying: moisture transition points control.
J Sci Food Agric
January 2025
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, China.
Background: Fresh Cistanche deserticola, a valuable medicinal and dietary ingredient with exceptional functional properties, exhibits a high moisture content and is susceptible to deterioration due to its high moisture content. Therefore, it is imperative to identify an appropriate dehydration method. This study primarily focuses on enhancing the efficiency and quality of dried C.
View Article and Find Full Text PDFEffect of Bovine Serum Albumin (BSA) Concentration on Cryopreservation of Booroolong Frog Sperm with Evaluation of Post-Thaw Motility in Caffeine.
Vet Sci
January 2025
Taronga Institute of Science and Learning, Taronga Conservation Society Australia, Mosman, NSW 2088, Australia.
Reproductive technologies, including sperm cryopreservation, offer conservationists enhanced capacity to genetically manage populations and improve the outcomes of conservation breeding programs (CBPs). Despite this potential, the post-thaw quality of amphibian sperm is highly variable following cryopreservation, and research focused on protocol refinement is needed. The aim of this study was twofold: (1) to investigate the effect of the addition of bovine serum albumin (BSA) to the cryopreservation medium (pre-freeze), and (2) the effect of the addition of caffeine to the activation medium (post-thaw), on post-thaw sperm characteristics in the critically endangered Booroolong frog ().
View Article and Find Full Text PDFThe Global Challenge of : Antimicrobial Resistance and Emerging Intervention Strategies.
Trop Med Infect Dis
January 2025
School of Health and Life Sciences, Teesside University, Middlesbrough TS1 3BX, UK.
Antimicrobial resistance (AMR) in species, particularly and , poses a significant public health threat. These bacteria, which are commonly found in livestock, poultry, companion animals, and wildlife, are the leading causes of foodborne illnesses, often transmitted through contaminated poultry. Extensive exposure to antibiotics in human and veterinary medicine creates selection pressure, driving resistance through mechanisms such as point mutations, horizontal gene transfer, and efflux pumps.
View Article and Find Full Text PDFEnzymatic Hydrolysis Systems Enhance the Efficiency and Biological Properties of Hydrolysates from Frozen Fish Processing Co-Products.
Mar Drugs
December 2024
Division of Aquaculture, Upgrading and Bioprospection, Portuguese Institute for the Sea and Atmosphere (IPMA, I.P.), Av. Doutor Alfredo Magalhães Ramalho 6, 1495-165 Algés, Portugal.
Co-products from the frozen fish processing industry often lead to financial losses. Therefore, it is essential to transform these co-products into profitable goods. This study explores the production of fish protein hydrolysates (FPH) from three co-products: the heads and bones of black scabbardfish (), the carcasses of gilthead seabream (), and the trimmings of Nile perch ().
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!